TY - JOUR
T1 - Characterization of Mesenchymal and Neural Stem Cells Response to Bipolar Microsecond Electric Pulses Stimulation
AU - Innamorati, Giorgia
AU - Sanchez-Petidier, Marina
AU - Bergafora, Giulia
AU - Codazzi, Camilla
AU - Palma, Valentina
AU - Camera, Francesca
AU - Merla, Caterina
AU - André, Franck M.
AU - Pedraza, Maria
AU - Moreno Manzano, Victoria
AU - Caramazza, Laura
AU - Colella, Micol
AU - Marracino, Paolo
AU - Balucani, Marco
AU - Apollonio, Francesca
AU - Liberti, Micaela
AU - Consales, Claudia
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2025/1/1
Y1 - 2025/1/1
N2 - In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.
AB - In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.
KW - cell proliferation
KW - gene expression
KW - microsecond electric pulse stimulation
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=85214522966&partnerID=8YFLogxK
U2 - 10.3390/ijms26010147
DO - 10.3390/ijms26010147
M3 - Article
AN - SCOPUS:85214522966
SN - 1661-6596
VL - 26
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 1
M1 - 147
ER -