Characterization of Mesenchymal and Neural Stem Cells Response to Bipolar Microsecond Electric Pulses Stimulation

Giorgia Innamorati, Marina Sanchez-Petidier, Giulia Bergafora, Camilla Codazzi, Valentina Palma, Francesca Camera, Caterina Merla, Franck M. André, Maria Pedraza, Victoria Moreno Manzano, Laura Caramazza, Micol Colella, Paolo Marracino, Marco Balucani, Francesca Apollonio, Micaela Liberti, Claudia Consales

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    Résumé

    In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that stem cell fate can be controlled by applying continuous electric field stimulation. This study aims to characterize the effect of a specific microsecond electric pulse stimulation (bipolar pulses of 100 µs + 100 µs, delivered for 30 min at an intensity of 250 V/cm) to induce an increase in cell proliferation on mesenchymal stem cells (MSCs) and induced neural stem cells (iNSCs). The effect was evaluated in terms of (i) cell counting, (ii) cell cycle, (iii) gene expression, and (iv) apoptosis. The results show that 24 h after the stimulation, cell proliferation, cell cycle, and apoptosis are not affected, but variation in the expression of specific genes involved in these processes is observed. These results led us to investigate cell proliferation until 72 h from the stimulation, observing an increase in the iNSCs number at this time point. The main outcome of this study is that the microsecond electric pulses can modulate stem cell proliferation.

    langue originaleAnglais
    Numéro d'article147
    journalInternational Journal of Molecular Sciences
    Volume26
    Numéro de publication1
    Les DOIs
    étatPublié - 1 janv. 2025

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