Characterization of Phytomonas sp. kinetoplast DNA. A plant pathogenic trypanosomal species

Jean François Riou, Michel Dollet, Jean Charles Ahomadegbe, Dominique Coulaud, Guy Riou

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    Résumé

    Phytomonas sp. which belongs to the Trypanosomatidae family, is a pathogen of plants. Its kinetoplast DNA consists of a huge network of about 7000 catenated minicircles of 2880 ± 30 base pairs each. It represents 30-33% of the total cellular DNA. An AT composition of 65% has been evaluated from the buoyant density xxx = 1.694 g ml. The restriction endonuclease HpaII was the only one able to cleave the minicircles into a single fragment. The other enzymes so far tested (EcoRI, HindIII, HaeIII, BamHI) do not cleave these minicircles significantly. Analysis of the kDNA by Southern blot hybridization shows that minicircles of Phytomonas sp. have little sequence homology with minicircles of other species of trypanosomes. Maxicircles, present in low proportions, have been identified. Our data indicate that the kinetoplast DNA of Phytomonas sp. presents biochemical characteristics which could be used to identify the Phytomonas genus.

    langue originaleAnglais
    Pages (de - à)304-308
    Nombre de pages5
    journalFEBS Letters
    Volume213
    Numéro de publication2
    Les DOIs
    étatPublié - 23 mars 1987

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