Chitosan-capped gold nanoparticles impair radioresistant glioblastoma stem-like cells

Mihaela Aldea, Monica Potara, Olga Soritau, Ioan Stefan Florian, Adrian Florea, Timea Nagy-Simon, Valentina Pileczki, Ioana Brie, Dana Maniu, Gabriel Kacso

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

8 Citations (Scopus)

Résumé

Purpose: Glioblastoma is a rapidly evolving lethal disease mainly due to its highly chemo- and radioresistant glioblastoma stem cells (GSCs). Herein, we tested if chitosan-capped gold nanoparticles (Chit-GNPs) may overcome the limitations of drug concentrations by increased cell internalization in GSCs and if such GNPs could enhance the response to irradiation. Methods: Chitosan was used for Chit-GNP synthesis as a reducing and stabilizing agent. Chit-GNPs were characterized by spectroscopy, dark field, transmission electron microscopy and zeta potential measurements. Patient-derived GSCs and human osteoblasts were treated with increasing concentrations of nanoparticles and irradiated. The uptake and cytotoxicity of Chit-GNPs were compared to that of uncoated GNPs. Results: The positively-charged, 26 nm-sized, spherical Chit-GNPs, showed a huge intracellular accumulation into the cytosol, lysosomes and near the nucleus, whereas no uncoated GNPs were internalized within GSCs. Surprisingly, Chit-GNPs were highly cytotoxic for GSCs irrespective of cell irradiation, that failed to add an additional benefit when combined with Chit-GNPs/GNPs. Moreover, Chit-GNPs were selectively cytotoxic for GSCs and did not affect the normal cells, despite an increased nanoparticle internalization. Conclusions: The important Chit-GNP internalization and their selective cytotoxicity for GSCs make this compound a potential novel anticancer agent and a promising backbone for drug delivery in glioblastoma.

langue originaleAnglais
Pages (de - à)800-813
Nombre de pages14
journalJournal of B.U.ON.
Volume23
Numéro de publication3
étatPublié - 1 mai 2018
Modification externeOui

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