TY - JOUR
T1 - Complement Mediated Signaling on Pulmonary CD103+ Dendritic Cells Is Critical for Their Migratory Function in Response to Influenza Infection
AU - Kandasamy, Matheswaran
AU - Ying, Poon C.
AU - Ho, Adrian W.S.
AU - Sumatoh, Hermi R.
AU - Schlitzer, Andreas
AU - Hughes, Timothy R.
AU - Kemeny, David M.
AU - Morgan, B. Paul
AU - Ginhoux, Florent
AU - Sivasankar, Baalasubramanian
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Trafficking of lung dendritic cells (DCs) to the draining lymph node (dLN) is a crucial step for the initiation of T cell responses upon pathogen challenge. However, little is known about the factors that regulate lung DC migration to the dLN. In this study, using a model of influenza infection, we demonstrate that complement component C3 is critically required for efficient emigration of DCs from the lung to the dLN. C3 deficiency affect lung DC-mediated viral antigen transport to the dLN, resulting in severely compromised priming of virus-specific T cell responses. Consequently, C3-deficient mice lack effector T cell response in the lungs that affected viral clearance and survival. We further show that direct signaling by C3a and C5a through C3aR and C5aR respectively expressed on lung DCs is required for their efficient trafficking. However, among lung DCs, only CD103+ DCs make a significant contribution to lung C5a levels and exclusively produce high levels of C3 and C5 during influenza infection. Collectively, our findings show that complement has a profound impact on immune regulation by controlling tissue DC trafficking and highlights a potential utility for complement as an adjuvant in novel vaccine strategies.
AB - Trafficking of lung dendritic cells (DCs) to the draining lymph node (dLN) is a crucial step for the initiation of T cell responses upon pathogen challenge. However, little is known about the factors that regulate lung DC migration to the dLN. In this study, using a model of influenza infection, we demonstrate that complement component C3 is critically required for efficient emigration of DCs from the lung to the dLN. C3 deficiency affect lung DC-mediated viral antigen transport to the dLN, resulting in severely compromised priming of virus-specific T cell responses. Consequently, C3-deficient mice lack effector T cell response in the lungs that affected viral clearance and survival. We further show that direct signaling by C3a and C5a through C3aR and C5aR respectively expressed on lung DCs is required for their efficient trafficking. However, among lung DCs, only CD103+ DCs make a significant contribution to lung C5a levels and exclusively produce high levels of C3 and C5 during influenza infection. Collectively, our findings show that complement has a profound impact on immune regulation by controlling tissue DC trafficking and highlights a potential utility for complement as an adjuvant in novel vaccine strategies.
UR - http://www.scopus.com/inward/record.url?scp=84875080678&partnerID=8YFLogxK
U2 - 10.1371/journal.ppat.1003115
DO - 10.1371/journal.ppat.1003115
M3 - Article
C2 - 23326231
AN - SCOPUS:84875080678
SN - 1553-7366
VL - 9
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 1
M1 - e1003115
ER -