TY - JOUR
T1 - Deep phenotyping of immune cell populations by optimized and standardized flow cytometry analyses
AU - Pitoiset, Fabien
AU - Cassard, Lydie
AU - El Soufi, Karim
AU - Boselli, Lisa
AU - Grivel, Jonathan
AU - Roux, Alexandra
AU - Klatzmann, David
AU - Chaput, Nathalie
AU - Rosenzwajg, Michelle
N1 - Publisher Copyright:
© 2018 International Society for Advancement of Cytometry
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Multicolor flow cytometry is a technology of choice for phenotyping of immune cells, and it can be used routinely for the follow up of patients in clinical trials. But it is challenging to define combinations of conjugated antibodies that efficiently allow the detailed analysis of major immune cell subsets and the identification of rare cell populations. In a collaborative work among the Immunology, Immunopathology, Immunotherapy (I3) laboratory, and the laboratory of immunomonitoring in oncology (L.I.O), we developed and validated 12 different 10-color flow cytometry panels that allow the deep immunophenotyping of cells from whole blood for the follow up of autoimmune and cancer patients. Here, we describe these optimized flow cytometry panels, showing that they provide the advanced analysis of T cells (including regulatory T cells), B cells, NK cells, MAIT cells, myeloid cells, monocytes, and dendritic cells. Most of the panels have been dried to improve standardization of the labeling and the entire procedure can be performed on less than 2 ml of whole blood. These deep immunophenotyping flow cytometry panels constitute a powerful tool for the monitoring of immune blood cells and will hopefully lead to the discovery of new biomarkers and potential therapeutic targets in autoimmune and cancer clinical trials.
AB - Multicolor flow cytometry is a technology of choice for phenotyping of immune cells, and it can be used routinely for the follow up of patients in clinical trials. But it is challenging to define combinations of conjugated antibodies that efficiently allow the detailed analysis of major immune cell subsets and the identification of rare cell populations. In a collaborative work among the Immunology, Immunopathology, Immunotherapy (I3) laboratory, and the laboratory of immunomonitoring in oncology (L.I.O), we developed and validated 12 different 10-color flow cytometry panels that allow the deep immunophenotyping of cells from whole blood for the follow up of autoimmune and cancer patients. Here, we describe these optimized flow cytometry panels, showing that they provide the advanced analysis of T cells (including regulatory T cells), B cells, NK cells, MAIT cells, myeloid cells, monocytes, and dendritic cells. Most of the panels have been dried to improve standardization of the labeling and the entire procedure can be performed on less than 2 ml of whole blood. These deep immunophenotyping flow cytometry panels constitute a powerful tool for the monitoring of immune blood cells and will hopefully lead to the discovery of new biomarkers and potential therapeutic targets in autoimmune and cancer clinical trials.
KW - clinical studies
KW - deep phenotyping
KW - flow cytometry
KW - human immunology
KW - immunomonitoring
KW - immunophenotyping
UR - http://www.scopus.com/inward/record.url?scp=85052812414&partnerID=8YFLogxK
U2 - 10.1002/cyto.a.23570
DO - 10.1002/cyto.a.23570
M3 - Article
C2 - 30168890
AN - SCOPUS:85052812414
SN - 1552-4922
VL - 93
SP - 793
EP - 802
JO - Cytometry Part A
JF - Cytometry Part A
IS - 8
ER -