TY - JOUR
T1 - Detection of anti-endoplasmic reticulum antibody-positive autoimmune hepatitis in children, using an ELISA technique
AU - Paradis, Khazal
AU - Dib, Amale
AU - Homberg, Jean Claude
AU - Bernard, Olivier
AU - Alagille, Daniel
AU - Alvarez, Fernando
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Anti-endoplasmic reticulum antibody positive autoimmune hepatitis in children is characterized by the recognition of a single 50.000 MW protein of the endo-plasmic reticulum in liver microsomal fractions by their sera. We have developed an enzyme-linked immunosorbent assay technique with rat liver microsomal preparations as the antigen to be used for detection of this disease, liters obtained may be useful in following the course of the disease and as an aid in determining when therapy can be discontinued. The technique is rapid, sensitive, reproducible, and simple to perform and is easier to manipulate than immunofluorescence or radioimmunoassay techniques.
AB - Anti-endoplasmic reticulum antibody positive autoimmune hepatitis in children is characterized by the recognition of a single 50.000 MW protein of the endo-plasmic reticulum in liver microsomal fractions by their sera. We have developed an enzyme-linked immunosorbent assay technique with rat liver microsomal preparations as the antigen to be used for detection of this disease, liters obtained may be useful in following the course of the disease and as an aid in determining when therapy can be discontinued. The technique is rapid, sensitive, reproducible, and simple to perform and is easier to manipulate than immunofluorescence or radioimmunoassay techniques.
KW - Anti-endoplasmic reticulum antibody positive autoimmune hepatitis
KW - Enzyme-linked immunosorbent assay
UR - http://www.scopus.com/inward/record.url?scp=0024120036&partnerID=8YFLogxK
U2 - 10.1097/00005176-198811000-00004
DO - 10.1097/00005176-198811000-00004
M3 - Article
C2 - 3058918
AN - SCOPUS:0024120036
SN - 0277-2116
VL - 7
SP - 816
EP - 822
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
IS - 6
ER -