TY - CHAP
T1 - Detection of gene rearrangements in circulating tumor cells
T2 - Examples of ALK-, ROS1-, RET-rearrangements in non-small-cell lung cancer and ERG-rearrangements in prostate cancer
AU - Catelain, Cyril
AU - Pailler, Emma
AU - Oulhen, Marianne
AU - Faugeroux, Vincent
AU - Pommier, Anne Laure
AU - Farace, Françoise
N1 - Publisher Copyright:
© Springer International Publishing AG 2017.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Circulating tumor cells (CTCs) hold promise as biomarkers to aid in patient treatment stratification and disease monitoring. Because the number of cells is a critical parameter for exploiting CTCs for predictive biomarker’s detection, we developed a FISH (fluorescent in situ hybridization) method for CTCs enriched on filters (filter-adapted FISH [FA-FISH]) that was optimized for high cell recovery. To increase the feasibility and reliability of the analyses, we combined fluorescent staining and FA-FISH and developed a semi-automated microscopy method for optimal FISH signal identification in filtration-enriched CTCs. Here we present these methods and their use for the detection and characterization of ALK-, ROS1-, RET-rearrangement in CTCs from non-small-cell lung cancer and ERG-rearrangements in CTCs from prostate cancer patients.
AB - Circulating tumor cells (CTCs) hold promise as biomarkers to aid in patient treatment stratification and disease monitoring. Because the number of cells is a critical parameter for exploiting CTCs for predictive biomarker’s detection, we developed a FISH (fluorescent in situ hybridization) method for CTCs enriched on filters (filter-adapted FISH [FA-FISH]) that was optimized for high cell recovery. To increase the feasibility and reliability of the analyses, we combined fluorescent staining and FA-FISH and developed a semi-automated microscopy method for optimal FISH signal identification in filtration-enriched CTCs. Here we present these methods and their use for the detection and characterization of ALK-, ROS1-, RET-rearrangement in CTCs from non-small-cell lung cancer and ERG-rearrangements in CTCs from prostate cancer patients.
KW - Circulating tumor cells
KW - FA-FISH
KW - Filtration-enrichment
KW - Fluorescent staining
KW - Predictive biomarkers
UR - http://www.scopus.com/inward/record.url?scp=85020022482&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-55947-6_9
DO - 10.1007/978-3-319-55947-6_9
M3 - Chapter
C2 - 28560674
AN - SCOPUS:85020022482
T3 - Advances in Experimental Medicine and Biology
SP - 169
EP - 179
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -