TY - JOUR
T1 - Differential upregulation of cellular adhesion molecules at the sites of oxidative stress in experimental acute pancreatitis
AU - Telek, Géza
AU - Ducroc, Robert
AU - Scoazec, Jean Yves
AU - Pasquier, Catherine
AU - Feldmann, Gérard
AU - Rozé, Claude
N1 - Funding Information:
This study was supported by grants from IRMAD Foundation France, Solvay Pharmaceuticals France, and Charles Debray Foundation, France. Confocal microscopy and image treatment facilities were kindly provided by INSERM Institut Fédératif de Recherches 02. The authors thank Dr. Andrew C. Issekutz, Dr. David Malka, and Dr. Barry A. Wolitzky for their generous gift of antibodies.
Funding Information:
1This study was supported in part by grants from IRMAD Foundation France, Solvay Pharmaceuticals France, and Charles Debray Foundation, France.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Background. Severe acute pancreatitis (AP) is associated with exaggerated leukocyte adherence and activation. Endothelial cellular adhesion molecules (CAMs) can be induced by cytokines, but also directly by oxygen free radicals (OFRs), mediated by nuclear factor kappa-B (NF-κB). We investigated the behavior of inducible CAMs in relation to pancreatic oxidative stress. Our novel modification of cerium capture histochemistry (reaction of OFRs with cerium produces laser reflective Ce perhydroxide precipitates) combined with reflectance confocal laser scanning microscopy (CLSM) allows the histological codemonstration of in vivo OFR production and immunolabeled CAMs, or NF-κB. Methods. Taurocholate AP was induced in rats; sham operated and normal animals served as controls. To achieve in situ, in vivo reaction of cerium with OFRs, animals were perfused with CeCl3 solution at different time points (1, 2, 8, 24 h) and then sacrificed. E-selectin, P-selectin, ICAM-1, VCAM, and NF-κB p65 were labeled by immunofluorescence (IF) on frozen sections of cerium perfused pancreata. IF and Ce perhydroxide reflectance were simultaneously detected by CLSM. Pancreatic gene expression of the same CAMs was quantified by competitive RT-PCR (MIMIC internal control). Results. Control pancreata showed negligible reflectance and minimal CAM expression. Early (1, 2 h) AP samples were characterized by intense, heterogeneous acinar OFR production, strong P-selectin, and increasing ICAM expression, with nuclear translocation of p65, histologically all colocalizing with the areas of acinar oxidative stress. Adherent polymorphonuclear leukocytes (PMNs) displayed weak OFR formation. Later (8, 24 h), a slowly declining P-selectin, but persisting ICAM-1 expression, was paralleled by widespread adherence of PMNs producing surprisingly large amounts of OFRs. VCAM and E-selectin showed a mild increase at 24 h. CAM gene activation was in good correlation with the protein expression. Conclusion. The early acinar oxidative stress is colocalized with NF-κB activation, preferential P-selectin, and ICAM upregulation in this AP model. Subsequently, adherent, activated PMNs become the major source of OFRs, thereby contributing to tissue damage.
AB - Background. Severe acute pancreatitis (AP) is associated with exaggerated leukocyte adherence and activation. Endothelial cellular adhesion molecules (CAMs) can be induced by cytokines, but also directly by oxygen free radicals (OFRs), mediated by nuclear factor kappa-B (NF-κB). We investigated the behavior of inducible CAMs in relation to pancreatic oxidative stress. Our novel modification of cerium capture histochemistry (reaction of OFRs with cerium produces laser reflective Ce perhydroxide precipitates) combined with reflectance confocal laser scanning microscopy (CLSM) allows the histological codemonstration of in vivo OFR production and immunolabeled CAMs, or NF-κB. Methods. Taurocholate AP was induced in rats; sham operated and normal animals served as controls. To achieve in situ, in vivo reaction of cerium with OFRs, animals were perfused with CeCl3 solution at different time points (1, 2, 8, 24 h) and then sacrificed. E-selectin, P-selectin, ICAM-1, VCAM, and NF-κB p65 were labeled by immunofluorescence (IF) on frozen sections of cerium perfused pancreata. IF and Ce perhydroxide reflectance were simultaneously detected by CLSM. Pancreatic gene expression of the same CAMs was quantified by competitive RT-PCR (MIMIC internal control). Results. Control pancreata showed negligible reflectance and minimal CAM expression. Early (1, 2 h) AP samples were characterized by intense, heterogeneous acinar OFR production, strong P-selectin, and increasing ICAM expression, with nuclear translocation of p65, histologically all colocalizing with the areas of acinar oxidative stress. Adherent polymorphonuclear leukocytes (PMNs) displayed weak OFR formation. Later (8, 24 h), a slowly declining P-selectin, but persisting ICAM-1 expression, was paralleled by widespread adherence of PMNs producing surprisingly large amounts of OFRs. VCAM and E-selectin showed a mild increase at 24 h. CAM gene activation was in good correlation with the protein expression. Conclusion. The early acinar oxidative stress is colocalized with NF-κB activation, preferential P-selectin, and ICAM upregulation in this AP model. Subsequently, adherent, activated PMNs become the major source of OFRs, thereby contributing to tissue damage.
UR - http://www.scopus.com/inward/record.url?scp=0035746585&partnerID=8YFLogxK
U2 - 10.1006/jsre.2000.6052
DO - 10.1006/jsre.2000.6052
M3 - Article
C2 - 11180997
AN - SCOPUS:0035746585
SN - 0022-4804
VL - 96
SP - 56
EP - 67
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -