TY - JOUR
T1 - Dual-mode gradient HPLC procedure for the simultaneous determination of chloroquine and proguanil
AU - Paci, A.
AU - Caire-Maurisier, A. M.
AU - Rieutord, A.
AU - Brion, F.
AU - Clair, P.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - In order to assay the antipaludic capsule of the Service de Santé des Armées (SSA), that contains two antimalarial drugs, i.e. chloroquine sulfate (CQS, cp1) and proguanil hydrochloride (PGH, cp5), a HPLC procedure was developed. A reversed-phase ion-pair high-performance liquid chromatography (HPLC) method with an ultraviolet detection at 254 nm was set up and validated. Elution system includes programming of both organic concentration and flow-rate known as 'dual-mode gradient'. This method allows the simultaneous determination of both active compounds and separation of four process related substances. The method is simple, rapid, selective and accurate, and the precision is good with an inter- and intra-assay of <2%. The sensitivity is particularly suitable for pharmaceutical quality control.
AB - In order to assay the antipaludic capsule of the Service de Santé des Armées (SSA), that contains two antimalarial drugs, i.e. chloroquine sulfate (CQS, cp1) and proguanil hydrochloride (PGH, cp5), a HPLC procedure was developed. A reversed-phase ion-pair high-performance liquid chromatography (HPLC) method with an ultraviolet detection at 254 nm was set up and validated. Elution system includes programming of both organic concentration and flow-rate known as 'dual-mode gradient'. This method allows the simultaneous determination of both active compounds and separation of four process related substances. The method is simple, rapid, selective and accurate, and the precision is good with an inter- and intra-assay of <2%. The sensitivity is particularly suitable for pharmaceutical quality control.
KW - Chloroquine sulfate
KW - Elution and flow-rate gradient
KW - HPLC-UV
KW - Proguanil hydrochloride
KW - Quality-control
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=0036027917&partnerID=8YFLogxK
U2 - 10.1016/S0731-7085(01)00555-6
DO - 10.1016/S0731-7085(01)00555-6
M3 - Article
C2 - 11682204
AN - SCOPUS:0036027917
SN - 0731-7085
VL - 27
SP - 1
EP - 7
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 1-2
ER -