Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior

Ioan Tivig, Leslie Vallet, Mihaela G. Moisescu, Romain Fernandes, Franck M. Andre, Lluis M. Mir, Tudor Savopol

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    Résumé

    The therapeutic use of mesenchymal stem cells (MSCs) becomes more and more important due to their potential for cell replacement procedures as well as due to their immunomodulatory properties. However, protocols for MSCs differentiation can be lengthy and may result in incomplete or asynchronous differentiation. To ensure homogeneous populations for therapeutic purposes, it is crucial to develop protocols for separation of the different cell types after differentiation. In this article we show that, when MSCs start to differentiate towards adipogenic or osteogenic progenies, their dielectrophoretic behavior changes. The values of cell electric parameters which can be obtained by dielectrophoretic measurements (membrane permittivity, conductivity, and cytoplasm conductivity) change before the morphological features of differentiation become microscopically visible. We further demonstrate, by simulation, that these electric modifications make possible to separate cells in their early stages of differentiation by using the dielectrophoretic separation technique. A label free method which allows obtaining cultures of homogenously differentiated cells is thus offered.

    langue originaleAnglais
    Numéro d'article4330
    journalScientific Reports
    Volume14
    Numéro de publication1
    Les DOIs
    étatPublié - 1 déc. 2024

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