TY - JOUR
T1 - Epithelial to mesenchymal transition and HPV infection in squamous cell oropharyngeal carcinomas
T2 - The papillophar study
AU - Papillophar Study Group
AU - Lefevre, Marine
AU - Rousseau, Alexandra
AU - Rayon, Thomas
AU - Dalstein, Véronique
AU - Clavel, Christine
AU - Beby-Defaux, Agnès
AU - Pretet, Jean Luc
AU - Soussan, Patrick
AU - Polette, Myriam
AU - Lacau Saint Guily, Jean
AU - Birembaut, Philippe
AU - Agius, G.
AU - Albert, S.
AU - Babin, E.
AU - Bach, C.
AU - Badet, J. M.
AU - Badoual, C.
AU - Baglin, A. C.
AU - Barry, B.
AU - Baujat, B.
AU - Bertolus, C.
AU - Blanc-Fournier, K.
AU - Cassagneau, E.
AU - Debry, C.
AU - De Raucourt, D.
AU - Diebold, M. D.
AU - Dufour, X.
AU - Hourseau, M.
AU - Kantelip, B.
AU - Lacave, R.
AU - Lechapt, E.
AU - Lerat, J.
AU - Lesnik, M.
AU - Levillain, P.
AU - Malard, O.
AU - Mechine, A.
AU - Merol, J. C.
AU - Mirghani, H.
AU - Morinière, S.
AU - Mougin, C.
AU - Périé, S.
AU - Rousselot, C.
AU - Schultz, P.
AU - Simon, T.
AU - Touzé, A.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Background: Human Papillomavirus (HPV) infection is recognised as aetiological factor of carcinogenesis in oropharyngeal squamous cell carcinomas (OPC). HPV-related OPC respond better to treatments and have a significantly favourable outcome. Epithelial to mesenchymal transition (EMT) implicated in tumour invasion, is a hallmark of a poor prognosis in carcinomas. Methods: We have studied the relationship of EMT markers (E-cadherin, β-catenin and vimentin) with HPV infection (DNA and E6/E7 mRNA detection), p16INK4a expression and survival outcomes in a cohort of 296 patients with OPC. Results: Among the 296 OPSSC, 26% were HPV positive, 20.3% had overt EMT (>25% of vimentin positive tumour cells). Lower E-cadherin expression was associated with a higher risk of distant metastasis in univariate (P=0.0110) and multivariate analyses (hazard ratios (HR)=6.86 (1.98; 23.84)). Vimentin expression tends towards worse metastasis-free survival (MFS; HR=2.53 (1.00; 6.41)) and was an independent prognostic factor of progression-free survival (HR=1.55 (1.03; 2.34)). Conclusions: There was a non significant association of EMT with HPV status. This may be explained by a mixed subpopulation of patients HPV positive with associated risk factors (HPV, tobacco and alcohol). Thus, the detection of EMT in OPC represents another reliable approach in the prognosis and the management of OPC whatever their HPV status.
AB - Background: Human Papillomavirus (HPV) infection is recognised as aetiological factor of carcinogenesis in oropharyngeal squamous cell carcinomas (OPC). HPV-related OPC respond better to treatments and have a significantly favourable outcome. Epithelial to mesenchymal transition (EMT) implicated in tumour invasion, is a hallmark of a poor prognosis in carcinomas. Methods: We have studied the relationship of EMT markers (E-cadherin, β-catenin and vimentin) with HPV infection (DNA and E6/E7 mRNA detection), p16INK4a expression and survival outcomes in a cohort of 296 patients with OPC. Results: Among the 296 OPSSC, 26% were HPV positive, 20.3% had overt EMT (>25% of vimentin positive tumour cells). Lower E-cadherin expression was associated with a higher risk of distant metastasis in univariate (P=0.0110) and multivariate analyses (hazard ratios (HR)=6.86 (1.98; 23.84)). Vimentin expression tends towards worse metastasis-free survival (MFS; HR=2.53 (1.00; 6.41)) and was an independent prognostic factor of progression-free survival (HR=1.55 (1.03; 2.34)). Conclusions: There was a non significant association of EMT with HPV status. This may be explained by a mixed subpopulation of patients HPV positive with associated risk factors (HPV, tobacco and alcohol). Thus, the detection of EMT in OPC represents another reliable approach in the prognosis and the management of OPC whatever their HPV status.
KW - epithelial to mesenchymal transition
KW - HPV
KW - oropharyngeal carcinoma
UR - http://www.scopus.com/inward/record.url?scp=85009168960&partnerID=8YFLogxK
U2 - 10.1038/bjc.2016.434
DO - 10.1038/bjc.2016.434
M3 - Article
C2 - 28072763
AN - SCOPUS:85009168960
SN - 0007-0920
VL - 116
SP - 362
EP - 369
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 3
ER -