Expression of DNA ligases I and II during oogenesis and early development of Xenopus laevis

Saïd Aoufouchi, Claude Prigent, Nadine Theze, Michel Philippe, Pierre Thiebaud

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

8 Citations (Scopus)

Résumé

We have analyzed the expression of DNA ligase I protein during oogenesis and early development of Xenopus laevis. The protein is already present in stage I oocytes and then accumulates throughout oogenesis to reach a steady state level by stage VI. It remains at this level at least until tadpole stage. In stage VI oocytes DNA ligase I protein is almost exclusively localized in the germinal vesicle. We have partially purified a DNA ligase II activity from stage VI oocytes, unfertilized eggs, and stage 8 embryos. An 80-kDa polypeptide can be specifically adenylated in all three purified extracts. It is not recognized by antibodies directed against DNA ligase I and is active on oligo(dT)-poly(rA) substrate. It could therefore represent DNA ligase II protein. The presence of both DNA ligases I and II in oocytes and embryos is inconsistent with the DNA ligase model that had been previously proposed for amphibia.

langue originaleAnglais
Pages (de - à)199-202
Nombre de pages4
journalDevelopmental Biology
Volume152
Numéro de publication1
Les DOIs
étatPublié - 1 janv. 1992
Modification externeOui

Contient cette citation