Filter-adapted fluorescent in situ hybridization (FA-FISH) for filtration-enriched circulating tumor cells

Marianne Oulhen; Emma Pailler; Vincent Faugeroux; Françoise Farace

doi:10.1007/978-1-4939-7144-2_10

Filter-adapted fluorescent in situ hybridization (FA-FISH) for filtration-enriched circulating tumor cells

Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

2 Citations (Scopus)

Résumé

Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.

langue originale	Anglais
titre	Methods in Molecular Biology
Editeur	Humana Press Inc.
Pages	133-141
Nombre de pages	9
Les DOIs	https://doi.org/10.1007/978-1-4939-7144-2_10
état	Publié - 1 janv. 2017

Série de publications

Nom	Methods in Molecular Biology
Volume	1634
ISSN (imprimé)	1064-3745

Accès au document

10.1007/978-1-4939-7144-2_10

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abstract = "Circulating tumor cells (CTCs) may represent an easily accessible source of tumor material to assess genetic aberrations such as gene-rearrangements or gene-amplifications and screen cancer patients eligible for targeted therapies. As the number of CTCs is a critical parameter to identify such biomarkers, we developed fluorescent in situ hybridization (FISH) for CTCs enriched on filters (filter-adapted-FISH, FA-FISH). Here, we describe the FA-FISH protocol, the combination of immunofluorescent staining (DAPI/CD45) and FA-FISH techniques, as well as the semi-automated microscopy method that we developed to improve the feasibility and reliability of FISH analyses in filtration-enriched CTC.",

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Filter-adapted fluorescent in situ hybridization (FA-FISH) for filtration-enriched circulating tumor cells. / Oulhen, Marianne; Pailler, Emma; Faugeroux, Vincent et al.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 133-141 (Methods in Molecular Biology; Vol 1634).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

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