Flow Cytometry Assessment of Lymphocyte Populations Infiltrating Liver Tumors

Maria Pérez-Lanzón, Céleste Plantureux, Juliette Paillet, Jules Sotty, Patrick Soussan, Guido Kroemer, Maria Chiara Maiuri, Jonathan Pol

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1 Citation (Scopus)

Résumé

Tissue-resident and recruited immune cells are essential mediators of natural and therapy-induced immunosurveillance of liver neoplasia. This idea has been recently reinforced by the clinical approval of immune checkpoint inhibitors for the immunotherapy of hepatocellular carcinoma and cholangiocarcinoma. Such research progress relies on the in-depth characterization of the immune populations that are present in pre-neoplastic and neoplastic hepatic lesions. A convenient technology for advancing along this path is high-dimensional cytometry. In this chapter, we present a protocol to assess the subtype and differentiation state of hepatic lymphocyte populations by multicolor immunofluorescence staining and flow cytometry. We detail the steps required for viability assessment and immune cell phenotyping of single-cell suspensions of liver cells by means of surface and intracellular staining of more than a dozen markers of interest. This protocol does not require prior removal of debris and dead cells and allows to process multiple samples in parallel. The procedure includes the use of a fixative-resistant viability dye that allows cell fixation and permeabilization after cell surface staining and before intracellular staining and data acquisition on a flow cytometer. Moreover, we provide a panel of fluorochrome-labeled antibodies designed for the characterization of lymphocytic subsets that can be adapted to distinct experimental settings. Finally, we present an overview of the post-staining pipeline, including data acquisition on a flow cytometer and tools for post-acquisition analyses.

langue originaleAnglais
titreMethods in Molecular Biology
EditeurHumana Press Inc.
Pages129-141
Nombre de pages13
Les DOIs
étatPublié - 1 janv. 2024
Modification externeOui

Série de publications

NomMethods in Molecular Biology
Volume2769
ISSN (imprimé)1064-3745
ISSN (Electronique)1940-6029

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