TY - JOUR
T1 - Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome
AU - Dawson, Simon
AU - Apcher, Sebastien
AU - Mee, Maureen
AU - Higashitsuji, Hiroaki
AU - Baker, Rohan
AU - Uhle, Stefan
AU - Dubiel, Wolfgang
AU - Fujita, Jun
AU - John Mayer, R.
PY - 2002/3/29
Y1 - 2002/3/29
N2 - A yeast two-hybrid screen with the human S6 (TBP7, RPT3) ATPase of the 26 S proteasome has identified gankyrin, a liver oncoprotein, as an interacting protein. Gankyrin interacts with both free and regulatory complex-associated S6 ATPase and is not stably associated with the 26 S particle. Deletional mutagenesis shows that the C-terminal 78 amino acids of the S6 ATPase are necessary and sufficient to mediate the interaction with gankyrin. Deletion of an orthologous gene in Saccharomyces cerevisiae suggests that it is dispensable for cell growth and viability. Overexpression and precipitation of tagged gankyrin from cultured cells detects a complex containing co-transfected tagged S6 ATPase (or endogenous S6) and endogenous cyclin D-dependent kinase CDK4. The proteasomal ATPases are part of the AAA (ATPases associated with diverse cellular activities) family, members of which are molecular chaperones; gankyrin complexes may therefore influence CDK4 function during oncogenesis.
AB - A yeast two-hybrid screen with the human S6 (TBP7, RPT3) ATPase of the 26 S proteasome has identified gankyrin, a liver oncoprotein, as an interacting protein. Gankyrin interacts with both free and regulatory complex-associated S6 ATPase and is not stably associated with the 26 S particle. Deletional mutagenesis shows that the C-terminal 78 amino acids of the S6 ATPase are necessary and sufficient to mediate the interaction with gankyrin. Deletion of an orthologous gene in Saccharomyces cerevisiae suggests that it is dispensable for cell growth and viability. Overexpression and precipitation of tagged gankyrin from cultured cells detects a complex containing co-transfected tagged S6 ATPase (or endogenous S6) and endogenous cyclin D-dependent kinase CDK4. The proteasomal ATPases are part of the AAA (ATPases associated with diverse cellular activities) family, members of which are molecular chaperones; gankyrin complexes may therefore influence CDK4 function during oncogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0037192842&partnerID=8YFLogxK
U2 - 10.1074/jbc.M107313200
DO - 10.1074/jbc.M107313200
M3 - Article
C2 - 11779854
AN - SCOPUS:0037192842
SN - 0021-9258
VL - 277
SP - 10893
EP - 10902
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -