GD3-7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase

Catherine Brenner; Bernhard Kniep; Evelyne Maillier; Cécile Martel; Claudia Franke; Nadja Röber; Michael Bachmann; Ernst Peter Rieber; Roger Sandhoff

doi:10.1016/j.bbrc.2009.11.044

GD3-7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase

Catherine Brenner, Bernhard Kniep, Evelyne Maillier, Cécile Martel, Claudia Franke, Nadja Röber, Michael Bachmann, Ernst Peter Rieber, Roger Sandhoff

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

11 Citations (Scopus)

Résumé

We prepared GD3-7-aldehyde (GD3-7) and determined its apoptotic potential. GD3-7 proved to be more efficient to induce pro-apoptotic mitochondrial alterations than GD3 when tested on mouse liver mitochondria. GD3-7-induced mitochondrial swelling and depolarization was blocked by cyclosporin A (CsA) supporting a critical role of the permeability transition pore complex (PTPC) during GD3-7-mediated apoptosis. In contrast to GD3, GD3-7 was able to induce channel formation in proteoliposomes containing adenine nucleotide translocase (ANT). This suggests that ANT is the molecular target of GD3-7. Using a specific antiserum, GD3-7 was detected in the lipid extract of the myeloid tumor cell line HL-60 after apoptosis induction, but not in living cells. Therefore, GD3-7 might be a novel mediator of PTPC-dependent apoptosis in cancer cells.

langue originale	Anglais
Pages (de - à)	248-253
Nombre de pages	6
journal	Biochemical and Biophysical Research Communications
Volume	391
Numéro de publication	1
Les DOIs	https://doi.org/10.1016/j.bbrc.2009.11.044
état	Publié - 1 janv. 2010
Modification externe	Oui

Accès au document

10.1016/j.bbrc.2009.11.044

Autres fichiers et liens

Lien vers la publication dans Scopus

Contient cette citation

@article{5a0604e0bda44591bb147c82e838e30b,

title = "GD3-7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase",

abstract = "We prepared GD3-7-aldehyde (GD3-7) and determined its apoptotic potential. GD3-7 proved to be more efficient to induce pro-apoptotic mitochondrial alterations than GD3 when tested on mouse liver mitochondria. GD3-7-induced mitochondrial swelling and depolarization was blocked by cyclosporin A (CsA) supporting a critical role of the permeability transition pore complex (PTPC) during GD3-7-mediated apoptosis. In contrast to GD3, GD3-7 was able to induce channel formation in proteoliposomes containing adenine nucleotide translocase (ANT). This suggests that ANT is the molecular target of GD3-7. Using a specific antiserum, GD3-7 was detected in the lipid extract of the myeloid tumor cell line HL-60 after apoptosis induction, but not in living cells. Therefore, GD3-7 might be a novel mediator of PTPC-dependent apoptosis in cancer cells.",

keywords = "Adenine nucleotide translocase, Apoptosis, GD3, GD3-7-aldehyde",

author = "Catherine Brenner and Bernhard Kniep and Evelyne Maillier and C{\'e}cile Martel and Claudia Franke and Nadja R{\"o}ber and Michael Bachmann and Rieber, {Ernst Peter} and Roger Sandhoff",

note = "Funding Information: C.B. is supported by grants funded by Association pour la Recherche sur le Cancer ( ARC ), EGIDE , l{\textquoteright}Agence Nationale pour la Recherche ( ANR , ANR-08PCVI-0008-01 ), and l{\textquoteright}Institut National pour le Cancer ( INCa , 2008-1-PL BIO-04-CNRS ON1 ). We thank Ms Christine Gr{\"a}fe and Ms Heidrun Gebauer for their help in preparing GD3-7 specific antisera. We are grateful to Britta Br{\"u}gger for making the triple Quadrupole nanoESI-MS/MS available to us. ",

year = "2010",

month = jan,

day = "1",

doi = "10.1016/j.bbrc.2009.11.044",

language = "English",

volume = "391",

pages = "248--253",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

number = "1",

}

TY - JOUR

T1 - GD3-7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase

AU - Brenner, Catherine

AU - Kniep, Bernhard

AU - Maillier, Evelyne

AU - Martel, Cécile

AU - Franke, Claudia

AU - Röber, Nadja

AU - Bachmann, Michael

AU - Rieber, Ernst Peter

AU - Sandhoff, Roger

N1 - Funding Information: C.B. is supported by grants funded by Association pour la Recherche sur le Cancer ( ARC ), EGIDE , l’Agence Nationale pour la Recherche ( ANR , ANR-08PCVI-0008-01 ), and l’Institut National pour le Cancer ( INCa , 2008-1-PL BIO-04-CNRS ON1 ). We thank Ms Christine Gräfe and Ms Heidrun Gebauer for their help in preparing GD3-7 specific antisera. We are grateful to Britta Brügger for making the triple Quadrupole nanoESI-MS/MS available to us.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - We prepared GD3-7-aldehyde (GD3-7) and determined its apoptotic potential. GD3-7 proved to be more efficient to induce pro-apoptotic mitochondrial alterations than GD3 when tested on mouse liver mitochondria. GD3-7-induced mitochondrial swelling and depolarization was blocked by cyclosporin A (CsA) supporting a critical role of the permeability transition pore complex (PTPC) during GD3-7-mediated apoptosis. In contrast to GD3, GD3-7 was able to induce channel formation in proteoliposomes containing adenine nucleotide translocase (ANT). This suggests that ANT is the molecular target of GD3-7. Using a specific antiserum, GD3-7 was detected in the lipid extract of the myeloid tumor cell line HL-60 after apoptosis induction, but not in living cells. Therefore, GD3-7 might be a novel mediator of PTPC-dependent apoptosis in cancer cells.

AB - We prepared GD3-7-aldehyde (GD3-7) and determined its apoptotic potential. GD3-7 proved to be more efficient to induce pro-apoptotic mitochondrial alterations than GD3 when tested on mouse liver mitochondria. GD3-7-induced mitochondrial swelling and depolarization was blocked by cyclosporin A (CsA) supporting a critical role of the permeability transition pore complex (PTPC) during GD3-7-mediated apoptosis. In contrast to GD3, GD3-7 was able to induce channel formation in proteoliposomes containing adenine nucleotide translocase (ANT). This suggests that ANT is the molecular target of GD3-7. Using a specific antiserum, GD3-7 was detected in the lipid extract of the myeloid tumor cell line HL-60 after apoptosis induction, but not in living cells. Therefore, GD3-7 might be a novel mediator of PTPC-dependent apoptosis in cancer cells.

KW - Adenine nucleotide translocase

KW - Apoptosis

KW - GD3

KW - GD3-7-aldehyde

UR - http://www.scopus.com/inward/record.url?scp=72949112225&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.11.044

DO - 10.1016/j.bbrc.2009.11.044

M3 - Article

C2 - 19912988

AN - SCOPUS:72949112225

SN - 0006-291X

VL - 391

SP - 248

EP - 253

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -