TY - JOUR
T1 - Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
AU - Probst, Hans Christian
AU - Stoitzner, Patrizia
AU - Amon, Lukas
AU - Backer, Ronald A.
AU - Brand, Anna
AU - Chen, Jianzhou
AU - Clausen, Björn E.
AU - Dieckmann, Sophie
AU - Dudziak, Diana
AU - Heger, Lukas
AU - Hodapp, Katrin
AU - Hornsteiner, Florian
AU - Hovav, Avi Hai
AU - Jacobi, Lukas
AU - Ji, Xingqi
AU - Kamenjarin, Nadine
AU - Lahl, Katharina
AU - Lahmar, Imran
AU - Lakus, Jelena
AU - Lehmann, Christian H.K.
AU - Ortner, Daniela
AU - Picard, Marion
AU - Roberti, Maria Paula
AU - Rossnagel, Lukas
AU - Saba, Yasmin
AU - Schalla, Carmen
AU - Schlitzer, Andreas
AU - Schraml, Barbara U.
AU - Schütze, Kristian
AU - Seichter, Anna
AU - Seré, Kristin
AU - Seretis, Athanasios
AU - Sopper, Sieghart
AU - Strandt, Helen
AU - Sykora, Martina M.
AU - Theobald, Hannah
AU - Tripp, Christoph H.
AU - Zitvogel, Laurence
N1 - Publisher Copyright:
© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.
PY - 2023/11/1
Y1 - 2023/11/1
N2 - This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
AB - This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
KW - Dendritic cells
KW - Discrimination of dendritic cell subsets
KW - Mouse nonlymphoid tissue
KW - Multiplex flow cytometry analysis
KW - Tissue digestion protocols
UR - http://www.scopus.com/inward/record.url?scp=85144063646&partnerID=8YFLogxK
U2 - 10.1002/eji.202249819
DO - 10.1002/eji.202249819
M3 - Article
AN - SCOPUS:85144063646
SN - 0014-2980
VL - 53
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 11
M1 - 2249819
ER -