High level of apoptosis and low AKT activation in mass screening as opposed to standard neuroblastoma

Hervé Sartelet, Shigeru Ohta, Stephane Barrette, Anne Laure Rougemont, Marie Brevet, Marie Regairaz, Isabelle Harvey, Chantal Bernard, Monique Fabre, Louis Gaboury, Luc L. Oligny, Jacques Bosq, Dominique Valteau-Couanet, Gilles Vassal

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    6 Citations (Scopus)

    Résumé

    Aims: Neuroblastoma is a paediatric solid tumour with a poor outcome except in children <1 year old. Based on catecholamine urinary excretion, mass screening (MS) programmes have been organized but failed to decrease the mortality of this tumour. To test the hypotheses of a spontaneous maturation/differentiation or regression, the levels of poly (ADP-ribose) polymerase (PARP)-1, an early apoptosis marker, of PhosphoAKT, a major apoptosis inhibitor, and of maturation/differentiation were compared in standard and in MS neuroblastomas. Methods and results: We performed a case-control study of 55 primary tumours and 21 metastases of MS neuroblastomas. Matched controls were standard unscreened neuroblastomas and were paired according to age, stage, and MYCN amplification. The tumours were included in tissue microarrays. Immunohistochemical staining was performed using antibodies against, AKT, phosphoAKT, TRKB and PARP-1. The expression of PARP-1 and that of phosphoAKT were significantly higher in standard than in MS neuroblastomas independently of age and stage of the tumour. PhosphoAKT and PARP-1 expression was significantly correlated in both tumours. Conclusions: These data suggest that the better prognosis of patients with MS neuroblastomas compared with classical neuroblastomas was secondary to spontaneous tumour regression mediated by higher levels of apoptosis associated with low activation of AKT.

    langue originaleAnglais
    Pages (de - à)607-616
    Nombre de pages10
    journalHistopathology
    Volume56
    Numéro de publication5
    Les DOIs
    étatPublié - 1 janv. 2010

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