High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

Jérôme Bouligand; Angelo Paci; Lionel Mercier; Gilles Vassal; Philippe Bourget

doi:10.1016/S0731-7085(03)00630-7

High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

Jérôme Bouligand, Angelo Paci, Lionel Mercier, Gilles Vassal, Philippe Bourget

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

23 Citations (Scopus)

Résumé

Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.

langue originale	Anglais
Pages (de - à)	525-530
Nombre de pages	6
journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	34
Numéro de publication	3
Les DOIs	https://doi.org/10.1016/S0731-7085(03)00630-7
état	Publié - 18 févr. 2004

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10.1016/S0731-7085(03)00630-7

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Bouligand, J., Paci, A., Mercier, L., Vassal, G., & Bourget, P. (2004). High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products. Journal of Pharmaceutical and Biomedical Analysis, 34(3), 525-530. https://doi.org/10.1016/S0731-7085(03)00630-7

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abstract = "Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.",

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Bouligand, J, Paci, A, Mercier, L, Vassal, G & Bourget, P 2004, 'High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products', Journal of Pharmaceutical and Biomedical Analysis, VOL. 34, Numéro 3, p. 525-530. https://doi.org/10.1016/S0731-7085(03)00630-7

High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products. / Bouligand, Jérôme; Paci, Angelo; Mercier, Lionel et al.
Dans: Journal of Pharmaceutical and Biomedical Analysis, Vol 34, Numéro 3, 18.02.2004, p. 525-530.

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

TY - JOUR

T1 - High-performance thin-layer chromatography with a derivatization procedure, a suitable method for the identification and the quantitation of busulfan in various pharmaceutical products

AU - Bouligand, Jérôme

AU - Paci, Angelo

AU - Mercier, Lionel

AU - Vassal, Gilles

AU - Bourget, Philippe

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AB - Busulfan is an alkylating agent widely used in combination chemotherapy regimens followed by allogeneic or autologous hematopoietic stem cell transplantation (HSCT). We present a rapid method for assaying busulfan in pharmaceutical preparations using high-performance thin-layer chromatography (HPTLC) and derivatization with 4-nitrobenzylpyridine. The method is accurate and precise and allows quantitation of busulfan in aqueous solutions from 100 to 500μg/ml. It is suitable for identification, quantitation and stability studies of busulfan in pharmaceutical products, i.e. capsules or infusion bags prepared in a hospital pharmacy.

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KW - Cyclophosphamide

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