TY - JOUR
T1 - HPV circulating tumoral DNA quantification by droplet-based digital PCR
T2 - A promising predictive and prognostic biomarker for HPV-associated oropharyngeal cancers
AU - Veyer, David
AU - Wack, Maxime
AU - Mandavit, Marion
AU - Garrigou, Sonia
AU - Hans, Stéphane
AU - Bonfils, Pierre
AU - Tartour, Eric
AU - Bélec, Laurent
AU - Wang-Renault, Shu Fang
AU - Laurent-Puig, Pierre
AU - Mirghani, Haitham
AU - Rance, Bastien
AU - Taly, Valérie
AU - Badoual, Cécile
AU - Péré, Hélène
N1 - Publisher Copyright:
© 2019 UICC
PY - 2020/8/15
Y1 - 2020/8/15
N2 - We aimed to determine whether pretherapeutic assessment of HPV circulating tumoral DNA (HPV ctDNA) by droplet-based digital PCR (ddPCR) could constitute a predictive and prognostic biomarker for HPV-associated oropharyngeal squamous cell carcinoma (OPSCC). A mono-institutional prospective biomarker study on 66 patients with p16+/HPV16-positive oropharyngeal squamous cell carcinoma (OPSCC) was conducted in European Georges Pompidou Hospital, Paris, France. Blood samples were collected at the time of diagnosis before any treatment. Optimized digital PCR assays were used to quantify HPV16 ctDNA. Forty-seven (71%) patients showed a positive pretherapeutic HPV ctDNA at time of diagnosis. Interestingly, the quantity of HPV16 ctDNA at baseline, as assessed by ddPCR, was significantly correlated with the T/N/M status or OPSCC stages according to the 2018 new staging criteria for high-risk human papillomavirus (HR HPV) related OPSCC from American Joint Committee on Cancer (AJCC). Moreover, all recurrences and the majority (83%) of death reported events occurred in patients with positive HPV16 ctDNA at baseline. Finally, when posttreatment blood samples were available (n = 6), the kinetic of pretreatment/posttreatment HPV16 ctDNA was clearly associated with treatment success or failure. HPV ctDNA monitoring by ddPCR could constitute a useful and noninvasive dynamic biomarker to select HR HPV-related OPSCC patients eligible for potential treatment de-escalation and to monitor treatment response.
AB - We aimed to determine whether pretherapeutic assessment of HPV circulating tumoral DNA (HPV ctDNA) by droplet-based digital PCR (ddPCR) could constitute a predictive and prognostic biomarker for HPV-associated oropharyngeal squamous cell carcinoma (OPSCC). A mono-institutional prospective biomarker study on 66 patients with p16+/HPV16-positive oropharyngeal squamous cell carcinoma (OPSCC) was conducted in European Georges Pompidou Hospital, Paris, France. Blood samples were collected at the time of diagnosis before any treatment. Optimized digital PCR assays were used to quantify HPV16 ctDNA. Forty-seven (71%) patients showed a positive pretherapeutic HPV ctDNA at time of diagnosis. Interestingly, the quantity of HPV16 ctDNA at baseline, as assessed by ddPCR, was significantly correlated with the T/N/M status or OPSCC stages according to the 2018 new staging criteria for high-risk human papillomavirus (HR HPV) related OPSCC from American Joint Committee on Cancer (AJCC). Moreover, all recurrences and the majority (83%) of death reported events occurred in patients with positive HPV16 ctDNA at baseline. Finally, when posttreatment blood samples were available (n = 6), the kinetic of pretreatment/posttreatment HPV16 ctDNA was clearly associated with treatment success or failure. HPV ctDNA monitoring by ddPCR could constitute a useful and noninvasive dynamic biomarker to select HR HPV-related OPSCC patients eligible for potential treatment de-escalation and to monitor treatment response.
KW - clinical outcome
KW - droplet-based digital PCR
KW - HPV OPSCC staging
KW - HPV16 ctDNA
UR - http://www.scopus.com/inward/record.url?scp=85077023338&partnerID=8YFLogxK
U2 - 10.1002/ijc.32804
DO - 10.1002/ijc.32804
M3 - Article
C2 - 31756275
AN - SCOPUS:85077023338
SN - 0020-7136
VL - 147
SP - 1222
EP - 1227
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 4
ER -