TY - JOUR
T1 - HPV detection and genotyping of head and neck cancer biopsies by molecular testing with regard to the new oropharyngeal squamous cell carcinoma classification based on HPV status
AU - Veyer, David
AU - Wack, Maxime
AU - Grard, Ophélie
AU - Bonfils, Pierre
AU - Hans, Stéphane
AU - Bélec, Laurent
AU - Badoual, Cécile
AU - Péré, Hélène
N1 - Publisher Copyright:
© 2019 Royal College of Pathologists of Australasia
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Recently, both the World Health Organization/International Agency for Research on Cancer (WHO/IARC)and the American Joint Committee on Cancer (AJCC)have classified oropharyngeal squamous cell carcinoma (OPSCC)on the basis of HPV status. For this purpose, the WHO/IARC recommended direct molecular HPV testing. In practice, formalin-fixed, paraffin-embedded (FFPE)biopsy specimens are frequently the only available samples. We herein compared in parallel two commercially available molecular assays that were first designed for cervical HPV detection and genotyping: Inno-Lipa HPV Genotyping Extra II (IL)and Anyplex II HPV28 (AP28). A total of 55 samples were tested. By IL assay, chosen as reference assay, 27 (49.1%)biopsies were positive for HPV16, 10 (18.2%)were positive for HPV but negative for HPV16, and 18 (32.7%)were negative for HPV. A valid result with AP28 was obtained for 51 biopsy samples (92.7%). Among 37 HPV positive samples by IL, 33 (89.2%)were positive by AP28. The agreement between both assays was good (Cohen's κ = 0.78). Among the six discrepancies between assays, always associated with low HPV16 viral load, four biopsies positive for HPV16 by IL could not be detected by AP28. Taken together, these observations demonstrate that both assays could be used in routine HPV detection and genotyping on FFPE biopsy samples of head and neck tumours.
AB - Recently, both the World Health Organization/International Agency for Research on Cancer (WHO/IARC)and the American Joint Committee on Cancer (AJCC)have classified oropharyngeal squamous cell carcinoma (OPSCC)on the basis of HPV status. For this purpose, the WHO/IARC recommended direct molecular HPV testing. In practice, formalin-fixed, paraffin-embedded (FFPE)biopsy specimens are frequently the only available samples. We herein compared in parallel two commercially available molecular assays that were first designed for cervical HPV detection and genotyping: Inno-Lipa HPV Genotyping Extra II (IL)and Anyplex II HPV28 (AP28). A total of 55 samples were tested. By IL assay, chosen as reference assay, 27 (49.1%)biopsies were positive for HPV16, 10 (18.2%)were positive for HPV but negative for HPV16, and 18 (32.7%)were negative for HPV. A valid result with AP28 was obtained for 51 biopsy samples (92.7%). Among 37 HPV positive samples by IL, 33 (89.2%)were positive by AP28. The agreement between both assays was good (Cohen's κ = 0.78). Among the six discrepancies between assays, always associated with low HPV16 viral load, four biopsies positive for HPV16 by IL could not be detected by AP28. Taken together, these observations demonstrate that both assays could be used in routine HPV detection and genotyping on FFPE biopsy samples of head and neck tumours.
KW - Anyplex II HPV28 HPV genotyping assay
KW - FFPE biopsy
KW - HNSCC
KW - HPV16 E6 viral load
KW - Inno-Lipa HPV Genotyping Extra II
UR - http://www.scopus.com/inward/record.url?scp=85064241037&partnerID=8YFLogxK
U2 - 10.1016/j.pathol.2019.02.002
DO - 10.1016/j.pathol.2019.02.002
M3 - Article
C2 - 31005250
AN - SCOPUS:85064241037
SN - 0031-3025
VL - 51
SP - 421
EP - 425
JO - Pathology
JF - Pathology
IS - 4
ER -