Human TCR α/β+ CD4-CD8- double-negative T cells in patients with autoimmune lymphoproliferative syndrome express restricted Vβ TCR diversity and are clonally related to CD8 + T cells

Anne Bristeau-Leprince, Véronique Mateo, Annick Lim, Aude Magerus-Chatinet, Eric Solary, Alain Fischer, Frédéric Rieux-Laucat, Marie Lise Gougeon

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

66 Citations (Scopus)

Résumé

The peripheral expansion of α/β+-CD4- CD8- double negative (DN) T cells in patients with autoimmune lymphoproliferative syndrome (ALPS) is a consistent feature of this disease, and part of the diagnostic criteria of ALPS. The origin of these cells remains undetermined. They could derive from mature T cells that have lost coreceptor expression, or represent a special minor cell lineage. To investigate relationship of DN and single positive (SP) T cells in ALPS, we used Immunoscope technology to analyze the TCRVβ repertoire diversity of sorted DN and SP T cells, and we performed CDR3 sequence analyses of matching clonotypes. We show that DN T cells express all the Vβ gene families that are used by their SP counterparts, though they dominantly use some Vβ genes. Analysis of CDR3 length distribution revealed a diverse polyclonal TCR repertoire for sorted CD4+ T cells, whereas both DN and CD8+ T cells showed a skewed TCR repertoire with oligoclonal expansions throughout most of the Vβ families. CDR3 sequencing of matching clonotypes revealed a significant sharing of CDR3 sequences from selected Vβ-Jβ transcripts between DN and CD8+ T cells. Altogether, these data strongly argue for a CD8 origin of DN T cells in ALPS.

langue originaleAnglais
Pages (de - à)440-448
Nombre de pages9
journalJournal of Immunology
Volume181
Numéro de publication1
Les DOIs
étatPublié - 1 janv. 2008
Modification externeOui

Contient cette citation