Hypermutation in human B cells in Vivo and in Vitro

Sandra Weller, Ahmad Faili, Said Aoufouchi, Quentin Guéranger, Moritz Braun, Claude Agnès Reynaud, Jean Claude Weill

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    23 Citations (Scopus)

    Résumé

    We develop our previous observation that a subpopulation of circulating memory IgM+IgD+CD27+ B cells belongs to a separate pathway of differentiation in humans. This subpopulation, which represents 5-25% of peripheral B cells, is also present in spleen in the same proportion and displays a marginal-zone-like B cell phenotype. In addition, we describe a short-time in vitro induction model for somatic hypermutation by using the BL2 Burkitt's lymphoma cell line stimulated by a combination of antibodies directed against different surface receptors. This short-time assay allows us to show that mutatious are stably introduced in one DNA strand of the BL2 VH gene in the G1 phase of the cell cycle.

    langue originaleAnglais
    Pages (de - à)158-165
    Nombre de pages8
    journalAnnals of the New York Academy of Sciences
    Volume987
    Les DOIs
    étatPublié - 1 janv. 2003

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