Résumé
We develop our previous observation that a subpopulation of circulating memory IgM+IgD+CD27+ B cells belongs to a separate pathway of differentiation in humans. This subpopulation, which represents 5-25% of peripheral B cells, is also present in spleen in the same proportion and displays a marginal-zone-like B cell phenotype. In addition, we describe a short-time in vitro induction model for somatic hypermutation by using the BL2 Burkitt's lymphoma cell line stimulated by a combination of antibodies directed against different surface receptors. This short-time assay allows us to show that mutatious are stably introduced in one DNA strand of the BL2 VH gene in the G1 phase of the cell cycle.
langue originale | Anglais |
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Pages (de - à) | 158-165 |
Nombre de pages | 8 |
journal | Annals of the New York Academy of Sciences |
Volume | 987 |
Les DOIs | |
état | Publié - 1 janv. 2003 |