TY - JOUR
T1 - Identification of a New Stromal Cell Type Involved in the Regulation of Inflamed B Cell Follicles
AU - Mionnet, Cyrille
AU - Mondor, Isabelle
AU - Jorquera, Audrey
AU - Loosveld, Marie
AU - Maurizio, Julien
AU - Arcangeli, Marie Laure
AU - Ruddle, Nancy H.
AU - Nowak, Jonathan
AU - Aurrand-Lions, Michel
AU - Luche, Hervé
AU - Bajénoff, Marc
PY - 2013/11/1
Y1 - 2013/11/1
N2 - Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We demonstrated that upon inflammation, B cell follicles progressively trespassed into the adjacent T cell zone and surrounded and converted these stromal cells into CXCL13 secreting cells that in return delineated the new boundaries of the growing follicle. Acute B cell ablation in inflamed LNs abolished CXCL13 secretion in these cells, while LT-β deficiency in B cells drastically affected this conversion. Altogether, we reveal the existence of a dormant stromal cell subset that can be functionally awakened by B cells to delineate the transient boundaries of their expanding territories upon inflammation.
AB - Lymph node (LN) stromal cells provide survival signals and adhesive substrata to lymphocytes. During an immune response, B cell follicles enlarge, questioning how LN stromal cells manage these cellular demands. Herein, we used a murine fate mapping system to describe a new stromal cell type that resides in the T cell zone of resting LNs. We demonstrated that upon inflammation, B cell follicles progressively trespassed into the adjacent T cell zone and surrounded and converted these stromal cells into CXCL13 secreting cells that in return delineated the new boundaries of the growing follicle. Acute B cell ablation in inflamed LNs abolished CXCL13 secretion in these cells, while LT-β deficiency in B cells drastically affected this conversion. Altogether, we reveal the existence of a dormant stromal cell subset that can be functionally awakened by B cells to delineate the transient boundaries of their expanding territories upon inflammation.
UR - http://www.scopus.com/inward/record.url?scp=84886606785&partnerID=8YFLogxK
U2 - 10.1371/journal.pbio.1001672
DO - 10.1371/journal.pbio.1001672
M3 - Article
C2 - 24130458
AN - SCOPUS:84886606785
SN - 1544-9173
VL - 11
JO - PLoS Biology
JF - PLoS Biology
IS - 10
M1 - e1001672
ER -