TY - JOUR
T1 - Identification of an IL-7-dependent pre-T committed population in the spleen
AU - Gautreau, Laetitia
AU - Arcangeli, Marie Laure
AU - Pasqualetto, Valérie
AU - Joret, Anne Marie
AU - Garcia-Cordier, Corinne
AU - Mégret, Jérôme
AU - Schneider, Elke
AU - Ezine, Sophie
PY - 2007/9/1
Y1 - 2007/9/1
N2 - Several extrathymic T cell progenitors have been described but their various contributions to the T cell lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin-Thy1.2+ T cell-committed population, rare in B6 mice, abundant in TCRα -/-, CD3ε-/-, and nude mice, and absent in IL-7- and Rag-2-deficient mice. Neither B nor myeloid cells are generated in vivo and in vitro. The incidence of these pre-T cells is under the control of thymus and/or mature T cells, as revealed by graft experiments. Indeed, IL-7 consumption by mature T cells inhibits the growth of these pre-T cells. Moreover, the nude spleen contains an additional Lin-Thy1.2+CD25+ subset which is detected in B6 mice only after thymectomy. We establish that the full pre-T cell potential and proliferation capacity are only present in the c-kitlow fraction of progenitors. We also show that most CCR9 + progenitors are retained in the spleen of nude mice, but present in the blood of B6 mice. Thus, our data describe a new T cell lineage restricted subset that accumulates in the spleen before migration to the thymus.
AB - Several extrathymic T cell progenitors have been described but their various contributions to the T cell lineage puzzle are unclear. In this study, we provide evidence for a splenic Lin-Thy1.2+ T cell-committed population, rare in B6 mice, abundant in TCRα -/-, CD3ε-/-, and nude mice, and absent in IL-7- and Rag-2-deficient mice. Neither B nor myeloid cells are generated in vivo and in vitro. The incidence of these pre-T cells is under the control of thymus and/or mature T cells, as revealed by graft experiments. Indeed, IL-7 consumption by mature T cells inhibits the growth of these pre-T cells. Moreover, the nude spleen contains an additional Lin-Thy1.2+CD25+ subset which is detected in B6 mice only after thymectomy. We establish that the full pre-T cell potential and proliferation capacity are only present in the c-kitlow fraction of progenitors. We also show that most CCR9 + progenitors are retained in the spleen of nude mice, but present in the blood of B6 mice. Thus, our data describe a new T cell lineage restricted subset that accumulates in the spleen before migration to the thymus.
UR - http://www.scopus.com/inward/record.url?scp=38449117211&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.179.5.2925
DO - 10.4049/jimmunol.179.5.2925
M3 - Article
C2 - 17709507
AN - SCOPUS:38449117211
SN - 0022-1767
VL - 179
SP - 2925
EP - 2935
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -