TY - JOUR
T1 - Identification of the main epitope on human cytochrome P450 IID6 recognized by anti-liver kidney microsome antibody
AU - Gueguen, Maryse
AU - Boniface, Odile
AU - Bernard, Olivier
AU - Clerc, François
AU - Cartwright, Terence
AU - Alvarez, Fernando
N1 - Funding Information:
We thank Soline Reboul for helpful technical advice. This work was supported by a grant from Caisse Nationale d’Assurance Maladie des Travailleurs Salaries (CNAMTS)/Institut National de la Sante et de la Recherche Medicale (INSERM).
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Antibodies present in the sera of a group of children with autoimmune hepatitis react with human cytochrome P450 IID6. cDNA constructions of various fragments of human P450 IID6 were made and expressed and the resulting peptides were tested in immunoblot with patients' sera. These allowed identification of at least two antigenic sites on the P450 molecule. The main one, recognized by all sera tested, is located between amino acids 239 and 271. Synthesis of three peptides covering this area of the molecule allowed identification of a sequence of three amino acids (tyrosine-tryptophane-asparagine) located at position 261-263 that constitutes the essential part of the epitope. A protein sequence data-base search revealed homologies between this region of human P450 and proteins from Salmonella typhimurium, from human T lymphotropic virus types 1 and 2 and Herpes simplex virus type 1.
AB - Antibodies present in the sera of a group of children with autoimmune hepatitis react with human cytochrome P450 IID6. cDNA constructions of various fragments of human P450 IID6 were made and expressed and the resulting peptides were tested in immunoblot with patients' sera. These allowed identification of at least two antigenic sites on the P450 molecule. The main one, recognized by all sera tested, is located between amino acids 239 and 271. Synthesis of three peptides covering this area of the molecule allowed identification of a sequence of three amino acids (tyrosine-tryptophane-asparagine) located at position 261-263 that constitutes the essential part of the epitope. A protein sequence data-base search revealed homologies between this region of human P450 and proteins from Salmonella typhimurium, from human T lymphotropic virus types 1 and 2 and Herpes simplex virus type 1.
UR - http://www.scopus.com/inward/record.url?scp=0025826359&partnerID=8YFLogxK
U2 - 10.1016/0896-8411(91)90180-K
DO - 10.1016/0896-8411(91)90180-K
M3 - Article
C2 - 1723273
AN - SCOPUS:0025826359
SN - 0896-8411
VL - 4
SP - 607
EP - 615
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
IS - 4
ER -