Imaging green fluorescent protein fusions in living fission yeast cells

P. T. Tran; Anne Paoletti; Fred Chang

doi:10.1016/j.ymeth.2003.11.017

Imaging green fluorescent protein fusions in living fission yeast cells

P. T. Tran, Anne Paoletti, Fred Chang

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

80 Citations (Scopus)

Résumé

The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.

langue originale	Anglais
Pages (de - à)	220-225
Nombre de pages	6
journal	Methods
Volume	33
Numéro de publication	3
Les DOIs	https://doi.org/10.1016/j.ymeth.2003.11.017
état	Publié - 1 juil. 2004
Modification externe	Oui

Accès au document

10.1016/j.ymeth.2003.11.017

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title = "Imaging green fluorescent protein fusions in living fission yeast cells",

abstract = "The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation, data acquisition, and image analysis appropriate for analysis of living cells.",

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KW - Digital imaging

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KW - GFP

KW - Microscope

KW - Microtubules

KW - Time-lapse

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