@inbook{36a5405a68a143c8a9f43bafac1e5c7e,
title = "Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri",
abstract = "Autophagy is an evolutionarily conserved biological process required for the turnover of the cytoplasm of eukaryotic cell. Beyond its catabolic nature, autophagy has a plethora of pro-survival functions, thus combatting hypoxia, nutrient shortage, and unfolded protein accumulation. Here, we introduce the naturally short-lived turquoise killifish Nothobranchius furzeri as an emerging model to study autophagic function in vivo, in response to environmental challenges. We show that starvation in killifish is sufficient to increase autophagic flux in the liver, thus enhancing the lipidation of microtubule-associated protein light chain 3 (LC3) and reducing the abundance of the autophagic substrate sequestosome-1 (SQSTM1). We describe an immunoblot-based comprehensive protocol to monitor fluctuations in autophagy in this model organism.",
keywords = "Autophagy, Killifish",
author = "Gerasimos Anagnostopoulos and L{\'e}a Mont{\'e}gut and Hui Chen and Vincent Carbonnier and Isabelle Martins and Khady Mangane and Abdelkrim Mannioui and Alex Bois and Baracco, {Elisa Elena} and Maiuri, {Maria Chiara} and Guido Kroemer",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
day = "1",
doi = "10.1016/bs.mcb.2020.10.007",
language = "English",
isbn = "9780128244876",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "123--138",
editor = "Oliver Kepp and Lorenzo Galluzzi",
booktitle = "Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B",
}