Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri

Gerasimos Anagnostopoulos, Léa Montégut, Hui Chen, Vincent Carbonnier, Isabelle Martins, Khady Mangane, Abdelkrim Mannioui, Alex Bois, Elisa Elena Baracco, Maria Chiara Maiuri, Guido Kroemer

    Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection!!ChapterRevue par des pairs

    Résumé

    Autophagy is an evolutionarily conserved biological process required for the turnover of the cytoplasm of eukaryotic cell. Beyond its catabolic nature, autophagy has a plethora of pro-survival functions, thus combatting hypoxia, nutrient shortage, and unfolded protein accumulation. Here, we introduce the naturally short-lived turquoise killifish Nothobranchius furzeri as an emerging model to study autophagic function in vivo, in response to environmental challenges. We show that starvation in killifish is sufficient to increase autophagic flux in the liver, thus enhancing the lipidation of microtubule-associated protein light chain 3 (LC3) and reducing the abundance of the autophagic substrate sequestosome-1 (SQSTM1). We describe an immunoblot-based comprehensive protocol to monitor fluctuations in autophagy in this model organism.

    langue originaleAnglais
    titreMonitoring Vesicular Trafficking in Cellular Responses to Stress - Part B
    rédacteurs en chefOliver Kepp, Lorenzo Galluzzi
    EditeurAcademic Press Inc.
    Pages123-138
    Nombre de pages16
    ISBN (imprimé)9780128244876
    Les DOIs
    étatPublié - 1 janv. 2021

    Série de publications

    NomMethods in Cell Biology
    Volume165
    ISSN (imprimé)0091-679X

    Contient cette citation