TY - JOUR
T1 - Immunohistochemichal expression of biomarkers
T2 - A comparative study between diagnostic bronchial biopsies and surgical specimens of non-small-cell lung cancer
AU - Taillade, L.
AU - Penault-Llorca, F.
AU - Boulet, T.
AU - Fouret, P.
AU - Michiels, S.
AU - Taranchon, E.
AU - Mountzios, G.
AU - Validire, P.
AU - Domont, J.
AU - Girard, P.
AU - Grunenwald, D.
AU - Le Chevalier, T.
AU - Soria, J. C.
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Background: The increasing use of biomarkers as molecular determinants of responsiveness to conventional chemotherapy or molecular targeted therapy has raised the question of the reliability and reproducibility of their evaluation in bronchial biopsies as compared with corresponding resected surgical specimens. Patients and methods: Immunohistochemical expression of five markers related to signal transduction [epidermal growth factor receptor (EGFR), phospho-Akt], cell proliferation (Ki-67), DNA repair [excision repair cross-complementing (ERCC)1] and cellular 'immortality' [human telomerase catalytic component (hTERT)], was assessed in 41 patients with operable non-small-cell lung cancer in both bronchial biopsies and whole surgical specimens. Results: High correlation coefficients were observed between the expression of ERCC1, hTERT and Ki-67 in the biopsies and the surgical specimens [0.83 (P < 0.0001); 0.55 (P < 0.001) and 0.64 (P < 0.0001), respectively]. On the other hand, biomarker expression in biopsy was less correlated with the expression in the whole tissue sample for the markers of signal response and transduction [0.24 (P = 0.17) and 0.29 (P = 0.09) for EGFR and phospho-Akt, respectively]. Conclusions: Our results indicate a lack of association in the expression of important biomarkers between lung biopsies and corresponding resected tumors, with discordance rates ranging between 9% and 41%. Although these results need to be further validated in larger cohorts, they indicate that the evaluation of the expression of biomarkers in bronchial biopsies can be misleading.
AB - Background: The increasing use of biomarkers as molecular determinants of responsiveness to conventional chemotherapy or molecular targeted therapy has raised the question of the reliability and reproducibility of their evaluation in bronchial biopsies as compared with corresponding resected surgical specimens. Patients and methods: Immunohistochemical expression of five markers related to signal transduction [epidermal growth factor receptor (EGFR), phospho-Akt], cell proliferation (Ki-67), DNA repair [excision repair cross-complementing (ERCC)1] and cellular 'immortality' [human telomerase catalytic component (hTERT)], was assessed in 41 patients with operable non-small-cell lung cancer in both bronchial biopsies and whole surgical specimens. Results: High correlation coefficients were observed between the expression of ERCC1, hTERT and Ki-67 in the biopsies and the surgical specimens [0.83 (P < 0.0001); 0.55 (P < 0.001) and 0.64 (P < 0.0001), respectively]. On the other hand, biomarker expression in biopsy was less correlated with the expression in the whole tissue sample for the markers of signal response and transduction [0.24 (P = 0.17) and 0.29 (P = 0.09) for EGFR and phospho-Akt, respectively]. Conclusions: Our results indicate a lack of association in the expression of important biomarkers between lung biopsies and corresponding resected tumors, with discordance rates ranging between 9% and 41%. Although these results need to be further validated in larger cohorts, they indicate that the evaluation of the expression of biomarkers in bronchial biopsies can be misleading.
KW - Biological markers
KW - Biopsy
KW - Correlation
KW - Immunohistochemistry
KW - Non-small-cell lung cancer
KW - Surgical specimen
UR - http://www.scopus.com/inward/record.url?scp=34447338821&partnerID=8YFLogxK
U2 - 10.1093/annonc/mdm072
DO - 10.1093/annonc/mdm072
M3 - Article
C2 - 17355950
AN - SCOPUS:34447338821
SN - 0923-7534
VL - 18
SP - 1043
EP - 1050
JO - Annals of Oncology
JF - Annals of Oncology
IS - 6
ER -