Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women

Fok Moon Lum; Vipin Narang; Susan Hue; Jie Chen; Naomi McGovern; Ravisankar Rajarethinam; Jeslin J.L. Tan; Siti Naqiah Amrun; Yi Hao Chan; Cheryl Y.P. Lee; Tze Kwang Chua; Wearn Xin Yee; Nicholas K.W. Yeo; Thiam Chye Tan; Xuan Liu; Sam Haldenby; Yee sin Leo; Florent Ginhoux; Jerry K.Y. Chan; Julian Hiscox; Chia Yin Chong; Lisa F.P. Ng

doi:10.1002/cti2.1082

Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women

Fok Moon Lum, Vipin Narang, Susan Hue, Jie Chen, Naomi McGovern, Ravisankar Rajarethinam, Jeslin J.L. Tan, Siti Naqiah Amrun, Yi Hao Chan, Cheryl Y.P. Lee, Tze Kwang Chua, Wearn Xin Yee, Nicholas K.W. Yeo, Thiam Chye Tan, Xuan Liu, Sam Haldenby, Yee sin Leo, Florent Ginhoux, Jerry K.Y. Chan, Julian HiscoxChia Yin Chong, Lisa F.P. Ng

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

18 Citations (Scopus)

Résumé

Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods: Full-term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results: In this study, each woman exhibited a unique immune response with raised IL-1RA, IP-10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co-localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus-infected pregnant patients.

langue originale	Anglais
Numéro d'article	e01082
journal	Clinical and Translational Immunology
Volume	8
Numéro de publication	11
Les DOIs	https://doi.org/10.1002/cti2.1082
état	Publié - 1 janv. 2019
Modification externe	Oui

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10.1002/cti2.1082

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Lum, F. M., Narang, V., Hue, S., Chen, J., McGovern, N., Rajarethinam, R., Tan, J. J. L., Amrun, S. N., Chan, Y. H., Lee, C. Y. P., Chua, T. K., Yee, W. X., Yeo, N. K. W., Tan, T. C., Liu, X., Haldenby, S., Leo, Y. S., Ginhoux, F., Chan, J. K. Y., ... Ng, L. F. P. (2019). Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women. Clinical and Translational Immunology, 8(11), Article e01082. https://doi.org/10.1002/cti2.1082

@article{91a64430b33e40c5832952e27ad54a70,

title = "Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women",

abstract = "Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods: Full-term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results: In this study, each woman exhibited a unique immune response with raised IL-1RA, IP-10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co-localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus-infected pregnant patients.",

keywords = "RNA-seq, Zika virus, histology, infection, placenta, transcriptomics",

author = "Lum, {Fok Moon} and Vipin Narang and Susan Hue and Jie Chen and Naomi McGovern and Ravisankar Rajarethinam and Tan, {Jeslin J.L.} and Amrun, {Siti Naqiah} and Chan, {Yi Hao} and Lee, {Cheryl Y.P.} and Chua, {Tze Kwang} and Yee, {Wearn Xin} and Yeo, {Nicholas K.W.} and Tan, {Thiam Chye} and Xuan Liu and Sam Haldenby and Leo, {Yee sin} and Florent Ginhoux and Chan, {Jerry K.Y.} and Julian Hiscox and Chong, {Chia Yin} and Ng, {Lisa F.P.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc.",

year = "2019",

month = jan,

day = "1",

doi = "10.1002/cti2.1082",

language = "English",

volume = "8",

journal = "Clinical and Translational Immunology",

issn = "2050-0068",

number = "11",

}

Lum, FM, Narang, V, Hue, S, Chen, J, McGovern, N, Rajarethinam, R, Tan, JJL, Amrun, SN, Chan, YH, Lee, CYP, Chua, TK, Yee, WX, Yeo, NKW, Tan, TC, Liu, X, Haldenby, S, Leo, YS, Ginhoux, F, Chan, JKY, Hiscox, J, Chong, CY & Ng, LFP 2019, 'Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women', Clinical and Translational Immunology, VOL. 8, Numéro 11, e01082. https://doi.org/10.1002/cti2.1082

TY - JOUR

T1 - Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women

AU - Lum, Fok Moon

AU - Narang, Vipin

AU - Hue, Susan

AU - Chen, Jie

AU - McGovern, Naomi

AU - Rajarethinam, Ravisankar

AU - Tan, Jeslin J.L.

AU - Amrun, Siti Naqiah

AU - Chan, Yi Hao

AU - Lee, Cheryl Y.P.

AU - Chua, Tze Kwang

AU - Yee, Wearn Xin

AU - Yeo, Nicholas K.W.

AU - Tan, Thiam Chye

AU - Liu, Xuan

AU - Haldenby, Sam

AU - Leo, Yee sin

AU - Ginhoux, Florent

AU - Chan, Jerry K.Y.

AU - Hiscox, Julian

AU - Chong, Chia Yin

AU - Ng, Lisa F.P.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods: Full-term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results: In this study, each woman exhibited a unique immune response with raised IL-1RA, IP-10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co-localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus-infected pregnant patients.

AB - Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear. Methods: Full-term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis. Results: In this study, each woman exhibited a unique immune response with raised IL-1RA, IP-10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co-localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies. Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus-infected pregnant patients.

KW - RNA-seq

KW - Zika virus

KW - histology

KW - infection

KW - placenta

KW - transcriptomics

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U2 - 10.1002/cti2.1082

DO - 10.1002/cti2.1082

M3 - Article

AN - SCOPUS:85075565647

SN - 2050-0068

VL - 8

JO - Clinical and Translational Immunology

JF - Clinical and Translational Immunology

IS - 11

M1 - e01082

ER -