Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota

Ahmad Faili, Said Aoufouchi, Eric Flatter, Quentin Guéranger, Claude Agnès Reynaud, Jean Claude Weill

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    180 Citations (Scopus)

    Résumé

    Somatic hypermutation of immunoglobulin genes is a unique, targeted, adaptive process. While B cells are engaged in germinal centres in T-dependent responses, single base substitutions are introduced in the expressed VH/VL genes to allow the selection of mutants with a higher affinity for the immunizing antigen. Almost every possible DNA transaction has been proposed to explain this process, but each of these models includes an errorprone DNA synthesis step that introduces the mutations1,2. The Y family of DNA polymerases3 - pol ν, pol ι, pol κ and rev1 - are specialized for copying DNA lesions and have high rates of error when copying a normal DNA template4,5. By performing gene inactivation in a Burkitt's lymphoma cell line inducible for hypermutation, we show here that somatic hypermutation is dependent on DNA polymerase iota.

    langue originaleAnglais
    Pages (de - à)944-947
    Nombre de pages4
    journalNature
    Volume419
    Numéro de publication6910
    Les DOIs
    étatPublié - 31 oct. 2002

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