TY - JOUR
T1 - Intra-thymic nurse cell lymphocytes can induce a graft-versus-host reaction with high efficiency
AU - Penninger, J.
AU - Klima, J.
AU - Kroemer, G.
AU - Dietrich, H.
AU - Hala, K.
AU - Wick, G.
N1 - Funding Information:
This work was supported by the Jubilaumsfonds of the Austrian National Bank (Grant No. 2784) and the Austrian Research Council (project No. S-41/05). The authors thank Dr G. Boeck for FACS analyses and Dr R. Kofler for constructive criticism, Mr E. Rainer and Mr W. Salvenmoser for excellent technical assistance, MS M. Ginzel and Mrs P. Muller for continuous support.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Thymic nurse cells (TNC) are multicellular complexes composed of morphologically intact thymocytes internalized within epithelial cells. Due to their unusual features, they have been suspected to provide an appropriate microenvironment for T-cell repertoire acquisition. In recent functional studies in the chicken system, we have shown that intra-thymic nurse cell- lymphocytes (TNC-L) are highly efficient inducers of a graft-versus-host reaction (GvHR) in an allogeneic chorionallantoic membrane (CAM) assay, manifested by the formation of visible colonies, so-called pocks. Immunohistochemical and electron microscopical assessment of pocks revealed no differences between the cellular components and ultrastructural features of GvHR induced by TNC-L or peripheral blood lymphocytes (PBL). In addition, the stimulation of this local GvHR by interleukin 2 (IL-2) was demonstrated.
AB - Thymic nurse cells (TNC) are multicellular complexes composed of morphologically intact thymocytes internalized within epithelial cells. Due to their unusual features, they have been suspected to provide an appropriate microenvironment for T-cell repertoire acquisition. In recent functional studies in the chicken system, we have shown that intra-thymic nurse cell- lymphocytes (TNC-L) are highly efficient inducers of a graft-versus-host reaction (GvHR) in an allogeneic chorionallantoic membrane (CAM) assay, manifested by the formation of visible colonies, so-called pocks. Immunohistochemical and electron microscopical assessment of pocks revealed no differences between the cellular components and ultrastructural features of GvHR induced by TNC-L or peripheral blood lymphocytes (PBL). In addition, the stimulation of this local GvHR by interleukin 2 (IL-2) was demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=0024394154&partnerID=8YFLogxK
U2 - 10.1016/0145-305X(89)90041-4
DO - 10.1016/0145-305X(89)90041-4
M3 - Article
C2 - 2806699
AN - SCOPUS:0024394154
SN - 0145-305X
VL - 13
SP - 313
EP - 327
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 4
ER -