Isolation of mouse Kupffer cells for phenotypic and functional studies

Francesco Andreata; Camille Blériot; Pietro Di Lucia; Giorgia De Simone; Valeria Fumagalli; Xenia Ficht; Cristian Gabriel Beccaria; Mirela Kuka; Florent Ginhoux; Matteo Iannacone

doi:10.1016/j.xpro.2021.100831

Isolation of mouse Kupffer cells for phenotypic and functional studies

Francesco Andreata, Camille Blériot, Pietro Di Lucia, Giorgia De Simone, Valeria Fumagalli, Xenia Ficht, Cristian Gabriel Beccaria, Mirela Kuka, Florent Ginhoux, Matteo Iannacone

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

12 Citations (Scopus)

Résumé

Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).

langue originale	Anglais
Numéro d'article	100831
journal	STAR Protocols
Volume	2
Numéro de publication	4
Les DOIs	https://doi.org/10.1016/j.xpro.2021.100831
état	Publié - 17 déc. 2021
Modification externe	Oui

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10.1016/j.xpro.2021.100831

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title = "Isolation of mouse Kupffer cells for phenotypic and functional studies",

abstract = "Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).",

keywords = "Cell culture, Cell isolation, Flow Cytometry/Mass Cytometry, Immunology, Single Cell",

author = "Francesco Andreata and Camille Bl{\'e}riot and {Di Lucia}, Pietro and {De Simone}, Giorgia and Valeria Fumagalli and Xenia Ficht and Beccaria, {Cristian Gabriel} and Mirela Kuka and Florent Ginhoux and Matteo Iannacone",

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T1 - Isolation of mouse Kupffer cells for phenotypic and functional studies

AU - Andreata, Francesco

AU - Blériot, Camille

AU - Di Lucia, Pietro

AU - De Simone, Giorgia

AU - Fumagalli, Valeria

AU - Ficht, Xenia

AU - Beccaria, Cristian Gabriel

AU - Kuka, Mirela

AU - Ginhoux, Florent

AU - Iannacone, Matteo

PY - 2021/12/17

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AB - Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).

KW - Cell culture

KW - Cell isolation

KW - Flow Cytometry/Mass Cytometry

KW - Immunology

KW - Single Cell

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AN - SCOPUS:85122832817

SN - 2666-1667

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JO - STAR Protocols

JF - STAR Protocols

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