TY - JOUR
T1 - Juvenile-onset recurrent respiratory papillomatosis aggressiveness
T2 - In situ study of the level of transcription of hpv e6 and e7
AU - Lépine, Charles
AU - Voron, Thibault
AU - Berrebi, Dominique
AU - Mandavit, Marion
AU - Nervo, Marine
AU - Outh-Gauer, Sophie
AU - Péré, Hélène
AU - Tournier, Louis
AU - Teissier, Natacha
AU - Tartour, Eric
AU - Leboulanger, Nicolas
AU - Galmiche, Louise
AU - Badoual, Cécile
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Juvenile-onset recurrent respiratory papillomatosis (JoRRP) is a condition related to HPV 6 and 11 infection which is characterized by the repeated growth of benign exophytic papilloma in the respiratory tract. Disease progression is unpredictable: some children experience minor symptoms, while others require multiple interventions due to florid growth. The aim of this study was to explore the biomarkers of JoRRP severity on a bicentric cohort of forty-eight children. We performed a CISH on the most recent sample of papilloma with a probe targeting the mRNA of the E6 and E7 genes of HPV 6 and 11 and an immunostaining with p16INK4a antibody. For each patient HPV RNA CISH staining was assessed semi-quantitatively to define two scores: 1+, defined as a low staining extent, and 2+, defined as a high staining extent. This series contained 19 patients with a score of 1+ and 29 with a score of 2+. Patients with a score of 2+ had a median of surgical excision (SE) per year that was twice that of patients with a score of 1+ (respectively 6.1 versus 2.8, p = 0.036). We found similar results with the median number of SE the first year. Regarding p16INK4a, all patients were negative. To conclude, HPV RNA CISH might be a biomarker which is predictive of disease aggressiveness in JoRRP, and might help in patient care management.
AB - Juvenile-onset recurrent respiratory papillomatosis (JoRRP) is a condition related to HPV 6 and 11 infection which is characterized by the repeated growth of benign exophytic papilloma in the respiratory tract. Disease progression is unpredictable: some children experience minor symptoms, while others require multiple interventions due to florid growth. The aim of this study was to explore the biomarkers of JoRRP severity on a bicentric cohort of forty-eight children. We performed a CISH on the most recent sample of papilloma with a probe targeting the mRNA of the E6 and E7 genes of HPV 6 and 11 and an immunostaining with p16INK4a antibody. For each patient HPV RNA CISH staining was assessed semi-quantitatively to define two scores: 1+, defined as a low staining extent, and 2+, defined as a high staining extent. This series contained 19 patients with a score of 1+ and 29 with a score of 2+. Patients with a score of 2+ had a median of surgical excision (SE) per year that was twice that of patients with a score of 1+ (respectively 6.1 versus 2.8, p = 0.036). We found similar results with the median number of SE the first year. Regarding p16INK4a, all patients were negative. To conclude, HPV RNA CISH might be a biomarker which is predictive of disease aggressiveness in JoRRP, and might help in patient care management.
KW - Chromogenic in situ hybridization
KW - HPV 11
KW - HPV 6
KW - Juvenile-onset recurrent respiratory papillomatosis
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=85091986545&partnerID=8YFLogxK
U2 - 10.3390/cancers12102836
DO - 10.3390/cancers12102836
M3 - Article
AN - SCOPUS:85091986545
SN - 2072-6694
VL - 12
SP - 1
EP - 12
JO - Cancers
JF - Cancers
IS - 10
M1 - 2836
ER -