TY - JOUR
T1 - Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with wilt diseases of coconut and oil palms have a two-domain structure
AU - Dollet, Michel
AU - Sturm, Nancy R.
AU - Ahomadegbe, Jean Charles
AU - Campbell, David A.
PY - 2001/11/27
Y1 - 2001/11/27
N2 - We report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two-domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart 1, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.
AB - We report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two-domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart 1, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.
KW - Conserved sequence block
KW - Hartrot
KW - Marchitez sorpressiva
KW - Plant trypanosomatid
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=0035960741&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(01)00448-7
DO - 10.1016/S0378-1097(01)00448-7
M3 - Article
C2 - 11728717
AN - SCOPUS:0035960741
SN - 0378-1097
VL - 205
SP - 65
EP - 69
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -