Lactation-associated macrophages exist in murine mammary tissue and human milk

Dilay Cansever, Ekaterina Petrova, Sinduya Krishnarajah, Caroline Mussak, Christina A. Welsh, Wiebke Mildenberger, Kevin Mulder, Victor Kreiner, Elsa Roussel, Sebastian A. Stifter, Myrto Andreadou, Pascale Zwicky, Nicole Puertas Jurado, Hubert Rehrauer, Ge Tan, Zhaoyuan Liu, Camille Blériot, Francesca Ronchi, Andrew J. Macpherson, Florent GinhouxGiancarlo Natalucci, Burkhard Becher, Melanie Greter

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

11 Citations (Scopus)

Résumé

Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c+CX3CR1+Dectin-1+ macrophage population (liMac) that was distinct from the two resident F4/80hi and F4/80lo macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. LiMacs developed independently of IL-34, but required CSF-1 signaling and were partly microbiota-dependent. Locally, they resided adjacent to the basal cells of the alveoli and extravasated into the milk. We found several macrophage subsets in human milk that resembled liMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.

langue originaleAnglais
Pages (de - à)1098-1109
Nombre de pages12
journalNature Immunology
Volume24
Numéro de publication7
Les DOIs
étatPublié - 1 juil. 2023
Modification externeOui

Contient cette citation