TY - JOUR
T1 - Lactation-associated macrophages exist in murine mammary tissue and human milk
AU - Cansever, Dilay
AU - Petrova, Ekaterina
AU - Krishnarajah, Sinduya
AU - Mussak, Caroline
AU - Welsh, Christina A.
AU - Mildenberger, Wiebke
AU - Mulder, Kevin
AU - Kreiner, Victor
AU - Roussel, Elsa
AU - Stifter, Sebastian A.
AU - Andreadou, Myrto
AU - Zwicky, Pascale
AU - Jurado, Nicole Puertas
AU - Rehrauer, Hubert
AU - Tan, Ge
AU - Liu, Zhaoyuan
AU - Blériot, Camille
AU - Ronchi, Francesca
AU - Macpherson, Andrew J.
AU - Ginhoux, Florent
AU - Natalucci, Giancarlo
AU - Becher, Burkhard
AU - Greter, Melanie
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/7/1
Y1 - 2023/7/1
N2 - Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c+CX3CR1+Dectin-1+ macrophage population (liMac) that was distinct from the two resident F4/80hi and F4/80lo macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. LiMacs developed independently of IL-34, but required CSF-1 signaling and were partly microbiota-dependent. Locally, they resided adjacent to the basal cells of the alveoli and extravasated into the milk. We found several macrophage subsets in human milk that resembled liMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.
AB - Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c+CX3CR1+Dectin-1+ macrophage population (liMac) that was distinct from the two resident F4/80hi and F4/80lo macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. LiMacs developed independently of IL-34, but required CSF-1 signaling and were partly microbiota-dependent. Locally, they resided adjacent to the basal cells of the alveoli and extravasated into the milk. We found several macrophage subsets in human milk that resembled liMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.
UR - http://www.scopus.com/inward/record.url?scp=85162100550&partnerID=8YFLogxK
U2 - 10.1038/s41590-023-01530-0
DO - 10.1038/s41590-023-01530-0
M3 - Article
C2 - 37337103
AN - SCOPUS:85162100550
SN - 1529-2908
VL - 24
SP - 1098
EP - 1109
JO - Nature Immunology
JF - Nature Immunology
IS - 7
ER -