TY - JOUR
T1 - Major T cell progenitor activity in bone marrow-derived spleen colonies
AU - Lancrin, Christophe
AU - Schneider, Elke
AU - Lambolez, Florence
AU - Arcangeli, Marie Laure
AU - Garcia-Cordier, Corinne
AU - Rocha, Benedita
AU - Ezine, Sophie
PY - 2002/4/1
Y1 - 2002/4/1
N2 - Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin-) subpopulations were distinguished, namely CD44+ Thy1- cells still capable of natural killer generation and transient low-level B cell generation, and T cell-restricted CD44- Thy1+ cells. At a molecular level, frequency of CD3ε and preTα mRNA was very different in each subset. Furthermore, only the CD44- Thy1+ subset have initiated rearrangements in the T cell receptor β locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.
AB - Common lymphoid progenitors (CLP) are generated in adult bone marrow (BM), but the intermediate steps leading to T cell commitment are unknown, and so is the site at which this commitment occurs. Here, we show that colonies arising in the spleen 12 days after BM injection harbor T cell precursors that are undetectable in BM. These precursors did not generate myeloid cells in vivo but repopulated the thymus and the peripheral T cell compartment much faster than did CLP. Two lineage negative (Lin-) subpopulations were distinguished, namely CD44+ Thy1- cells still capable of natural killer generation and transient low-level B cell generation, and T cell-restricted CD44- Thy1+ cells. At a molecular level, frequency of CD3ε and preTα mRNA was very different in each subset. Furthermore, only the CD44- Thy1+ subset have initiated rearrangements in the T cell receptor β locus. Thus, this study identifies extramedullary T cell progenitors and will allow easy approach to T cell commitment studies.
KW - CFU-S
KW - Commitment
KW - Lymphoid differentiation
KW - Thymus
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0036535493&partnerID=8YFLogxK
U2 - 10.1084/jem.20011475
DO - 10.1084/jem.20011475
M3 - Article
C2 - 11927635
AN - SCOPUS:0036535493
SN - 0022-1007
VL - 195
SP - 919
EP - 929
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 7
ER -