Mapping the human DC lineage through the integration of high-dimensional techniques

Peter See; Charles Antoine Dutertre; Jinmiao Chen; Patrick Günther; Naomi McGovern; Sergio Erdal Irac; Merry Gunawan; Marc Beyer; Kristian Händler; Kaibo Duan; Hermi Rizal Bin Sumatoh; Nicolas Ruffin; Mabel Jouve; Ester Gea-Mallorquí; Raoul C.M. Hennekam; Tony Lim; Chan Chung Yip; Ming Wen; Benoit Malleret; Ivy Low; Nurhidaya Binte Shadan; Charlene Foong Shu Fen; Alicia Tay; Josephine Lum; Francesca Zolezzi; Anis Larbi; Michael Poidinger; Jerry K.Y. Chan; Qingfeng Chen; Laurent Rénia; Muzlifah Haniffa; Philippe Benaroch; Andreas Schlitzer; Joachim L. Schultze; Evan W. Newell; Florent Ginhoux

doi:10.1126/science.aag3009

Mapping the human DC lineage through the integration of high-dimensional techniques

Peter See, Charles Antoine Dutertre, Jinmiao Chen, Patrick Günther, Naomi McGovern, Sergio Erdal Irac, Merry Gunawan, Marc Beyer, Kristian Händler, Kaibo Duan, Hermi Rizal Bin Sumatoh, Nicolas Ruffin, Mabel Jouve, Ester Gea-Mallorquí, Raoul C.M. Hennekam, Tony Lim, Chan Chung Yip, Ming Wen, Benoit Malleret, Ivy LowNurhidaya Binte Shadan, Charlene Foong Shu Fen, Alicia Tay, Josephine Lum, Francesca Zolezzi, Anis Larbi, Michael Poidinger, Jerry K.Y. Chan, Qingfeng Chen, Laurent Rénia, Muzlifah Haniffa, Philippe Benaroch, Andreas Schlitzer, Joachim L. Schultze, Evan W. Newell, Florent Ginhoux

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

410 Citations (Scopus)

Résumé

Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.

langue originale	Anglais
Numéro d'article	eaag3009
journal	Science
Volume	356
Numéro de publication	6342
Les DOIs	https://doi.org/10.1126/science.aag3009
état	Publié - 9 juin 2017
Modification externe	Oui

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10.1126/science.aag3009

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See, P., Dutertre, C. A., Chen, J., Günther, P., McGovern, N., Irac, S. E., Gunawan, M., Beyer, M., Händler, K., Duan, K., Bin Sumatoh, H. R., Ruffin, N., Jouve, M., Gea-Mallorquí, E., Hennekam, R. C. M., Lim, T., Yip, C. C., Wen, M., Malleret, B., ... Ginhoux, F. (2017). Mapping the human DC lineage through the integration of high-dimensional techniques. Science, 356(6342), Article eaag3009. https://doi.org/10.1126/science.aag3009

@article{442866ba958f453d8af966f3ce86fe80,

title = "Mapping the human DC lineage through the integration of high-dimensional techniques",

abstract = "Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.",

author = "Peter See and Dutertre, {Charles Antoine} and Jinmiao Chen and Patrick G{\"u}nther and Naomi McGovern and Irac, {Sergio Erdal} and Merry Gunawan and Marc Beyer and Kristian H{\"a}ndler and Kaibo Duan and {Bin Sumatoh}, {Hermi Rizal} and Nicolas Ruffin and Mabel Jouve and Ester Gea-Mallorqu{\'i} and Hennekam, {Raoul C.M.} and Tony Lim and Yip, {Chan Chung} and Ming Wen and Benoit Malleret and Ivy Low and Shadan, {Nurhidaya Binte} and Fen, {Charlene Foong Shu} and Alicia Tay and Josephine Lum and Francesca Zolezzi and Anis Larbi and Michael Poidinger and Chan, {Jerry K.Y.} and Qingfeng Chen and Laurent R{\'e}nia and Muzlifah Haniffa and Philippe Benaroch and Andreas Schlitzer and Schultze, {Joachim L.} and Newell, {Evan W.} and Florent Ginhoux",

year = "2017",

month = jun,

day = "9",

doi = "10.1126/science.aag3009",

language = "English",

volume = "356",

journal = "Science",

issn = "0036-8075",

number = "6342",

}

See, P, Dutertre, CA, Chen, J, Günther, P, McGovern, N, Irac, SE, Gunawan, M, Beyer, M, Händler, K, Duan, K, Bin Sumatoh, HR, Ruffin, N, Jouve, M, Gea-Mallorquí, E, Hennekam, RCM, Lim, T, Yip, CC, Wen, M, Malleret, B, Low, I, Shadan, NB, Fen, CFS, Tay, A, Lum, J, Zolezzi, F, Larbi, A, Poidinger, M, Chan, JKY, Chen, Q, Rénia, L, Haniffa, M, Benaroch, P, Schlitzer, A, Schultze, JL, Newell, EW & Ginhoux, F 2017, 'Mapping the human DC lineage through the integration of high-dimensional techniques', Science, VOL. 356, Numéro 6342, eaag3009. https://doi.org/10.1126/science.aag3009

TY - JOUR

T1 - Mapping the human DC lineage through the integration of high-dimensional techniques

AU - See, Peter

AU - Dutertre, Charles Antoine

AU - Chen, Jinmiao

AU - Günther, Patrick

AU - McGovern, Naomi

AU - Irac, Sergio Erdal

AU - Gunawan, Merry

AU - Beyer, Marc

AU - Händler, Kristian

AU - Duan, Kaibo

AU - Bin Sumatoh, Hermi Rizal

AU - Ruffin, Nicolas

AU - Jouve, Mabel

AU - Gea-Mallorquí, Ester

AU - Hennekam, Raoul C.M.

AU - Lim, Tony

AU - Yip, Chan Chung

AU - Wen, Ming

AU - Malleret, Benoit

AU - Low, Ivy

AU - Shadan, Nurhidaya Binte

AU - Fen, Charlene Foong Shu

AU - Tay, Alicia

AU - Lum, Josephine

AU - Zolezzi, Francesca

AU - Larbi, Anis

AU - Poidinger, Michael

AU - Chan, Jerry K.Y.

AU - Chen, Qingfeng

AU - Rénia, Laurent

AU - Haniffa, Muzlifah

AU - Benaroch, Philippe

AU - Schlitzer, Andreas

AU - Schultze, Joachim L.

AU - Newell, Evan W.

AU - Ginhoux, Florent

PY - 2017/6/9

Y1 - 2017/6/9

N2 - Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.

AB - Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies-single-cell messenger RNA sequencing (scmRNAseq) and cytometry by time-of-flight (CyTOF)-to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.

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U2 - 10.1126/science.aag3009

DO - 10.1126/science.aag3009

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VL - 356

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