TY - JOUR
T1 - MGL_3741 gene contributes to pathogenicity of Malassezia globosa in pityriasis versicolor
AU - Aghaei Gharehbolagh, Sanaz
AU - Kordbacheh, Parivash
AU - Hashemi, Seyed Jamal
AU - Daie Ghazvini, Roshanak
AU - Asgari, Yazdan
AU - Agha Kuchak Afshari, Setareh
AU - Seyedmousavi, Seyedmojtaba
AU - Rezaie, Sassan
N1 - Publisher Copyright:
© 2018 Blackwell Verlag GmbH
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Dihydroxyacid dehydratase (DHAD) is a key enzyme in biosynthetic pathway of isoleucine and valine. This pathway is absent in human but exists in various organisms such as fungi. Using RNA-seq analysis in this study, we identified MGL_3741gene which encodes DHAD protein in Malassezia globosa (M. globosa). Furthermore, we found that mentioned gene is homologous to the Ustilago maydis, Saccharomyces cerevisiae, Aspergillus flavus, and Aspergillus fumigatus ILV3P. For understanding the probable role of this gene in pathogenicity of M. globosa, we applied Real-time PCR to investigate the differentially expressed of the MGL_3741 gene in healthy and pathogenic states. Our results indicate a significant difference between two mentioned stats. These results revealed that ILV3-like gene in M. globosa can be related to the pathogenicity of this yeast.
AB - Dihydroxyacid dehydratase (DHAD) is a key enzyme in biosynthetic pathway of isoleucine and valine. This pathway is absent in human but exists in various organisms such as fungi. Using RNA-seq analysis in this study, we identified MGL_3741gene which encodes DHAD protein in Malassezia globosa (M. globosa). Furthermore, we found that mentioned gene is homologous to the Ustilago maydis, Saccharomyces cerevisiae, Aspergillus flavus, and Aspergillus fumigatus ILV3P. For understanding the probable role of this gene in pathogenicity of M. globosa, we applied Real-time PCR to investigate the differentially expressed of the MGL_3741 gene in healthy and pathogenic states. Our results indicate a significant difference between two mentioned stats. These results revealed that ILV3-like gene in M. globosa can be related to the pathogenicity of this yeast.
KW - ILV3-like gene
KW - Malassezia globosa
KW - RNA-seq
UR - http://www.scopus.com/inward/record.url?scp=85053689622&partnerID=8YFLogxK
U2 - 10.1111/myc.12840
DO - 10.1111/myc.12840
M3 - Article
C2 - 30106184
AN - SCOPUS:85053689622
SN - 0933-7407
VL - 61
SP - 938
EP - 944
JO - Mycoses
JF - Mycoses
IS - 12
ER -