Monitoring SPB biogenesis in fission yeast with high resolution and quantitative fluorescent microscopy

Imene B. Bouhlel; Kathleen Scheffler; Phong T. Tran; Anne Paoletti

doi:10.1016/bs.mcb.2015.03.005

Monitoring SPB biogenesis in fission yeast with high resolution and quantitative fluorescent microscopy

Imene B. Bouhlel, Kathleen Scheffler, Phong T. Tran, Anne Paoletti

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Conference contribution › Revue par des pairs

1 Citation (Scopus)

Résumé

Like centrosomes, yeast spindle pole bodies (SPBs) undergo a tightly controlled duplication cycle in order to restrict their number to one or two per cell and promote the assembly of a bipolar spindle at mitotic entry. This conservative duplication cycle is tightly coordinated with cell cycle progression although the mechanisms that ensure this coordination remain largely unknown. In this chapter, we describe simple high resolution microscopy- and quantitative light microscopy-based methods that allow to monitor SPB biogenesis in fission yeast and may be useful to study the molecular pathways controlling the successive phases of the duplication cycle.

langue originale	Anglais
titre	Methods in Cell Biology
Sous-titre	Centrosome
rédacteurs en chef	Karen Oegema, Renata Basto
Editeur	Academic Press Inc.
Pages	383-392
Nombre de pages	10
ISBN (imprimé)	9780128024492
Les DOIs	https://doi.org/10.1016/bs.mcb.2015.03.005
état	Publié - 1 janv. 2015
Modification externe	Oui

Série de publications

Nom	Methods in Cell Biology
Volume	129
ISSN (imprimé)	0091-679X

Accès au document

10.1016/bs.mcb.2015.03.005

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Lien vers la publication dans Scopus

Contient cette citation

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title = "Monitoring SPB biogenesis in fission yeast with high resolution and quantitative fluorescent microscopy",

abstract = "Like centrosomes, yeast spindle pole bodies (SPBs) undergo a tightly controlled duplication cycle in order to restrict their number to one or two per cell and promote the assembly of a bipolar spindle at mitotic entry. This conservative duplication cycle is tightly coordinated with cell cycle progression although the mechanisms that ensure this coordination remain largely unknown. In this chapter, we describe simple high resolution microscopy- and quantitative light microscopy-based methods that allow to monitor SPB biogenesis in fission yeast and may be useful to study the molecular pathways controlling the successive phases of the duplication cycle.",

author = "Bouhlel, {Imene B.} and Kathleen Scheffler and Tran, {Phong T.} and Anne Paoletti",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

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doi = "10.1016/bs.mcb.2015.03.005",

language = "English",

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Monitoring SPB biogenesis in fission yeast with high resolution and quantitative fluorescent microscopy. / Bouhlel, Imene B.; Scheffler, Kathleen; Tran, Phong T. et al.
Methods in Cell Biology: Centrosome. Ed. / Karen Oegema; Renata Basto. Academic Press Inc., 2015. p. 383-392 (Methods in Cell Biology; Vol 129).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Conference contribution › Revue par des pairs

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N2 - Like centrosomes, yeast spindle pole bodies (SPBs) undergo a tightly controlled duplication cycle in order to restrict their number to one or two per cell and promote the assembly of a bipolar spindle at mitotic entry. This conservative duplication cycle is tightly coordinated with cell cycle progression although the mechanisms that ensure this coordination remain largely unknown. In this chapter, we describe simple high resolution microscopy- and quantitative light microscopy-based methods that allow to monitor SPB biogenesis in fission yeast and may be useful to study the molecular pathways controlling the successive phases of the duplication cycle.

AB - Like centrosomes, yeast spindle pole bodies (SPBs) undergo a tightly controlled duplication cycle in order to restrict their number to one or two per cell and promote the assembly of a bipolar spindle at mitotic entry. This conservative duplication cycle is tightly coordinated with cell cycle progression although the mechanisms that ensure this coordination remain largely unknown. In this chapter, we describe simple high resolution microscopy- and quantitative light microscopy-based methods that allow to monitor SPB biogenesis in fission yeast and may be useful to study the molecular pathways controlling the successive phases of the duplication cycle.

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