Monitoring TFEB translocation

Guo Chen; Chenglong Mu; Yanfang Chen; Na An; Yushan Zhu; Oliver Kepp; Guido Kroemer

doi:10.1016/bs.mcb.2020.10.017

Monitoring TFEB translocation

Guo Chen, Chenglong Mu, Yanfang Chen, Na An, Yushan Zhu, Oliver Kepp, Guido Kroemer

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

4 Citations (Scopus)

Résumé

The transcription factor EB (TFEB) plays a critical role in autophagy induction and lysosomal biogenesis by orchestrating the expression of autophagy- and lysosome-related genes. In response to a series of stresses such as nutrient starvation, TFEB translocates from the cytoplasm to the nucleus, where it exerts its regulatory function. The activity of TFEB is tightly regulated by multiple phosphorylation and acetylation sites. Methods that rely on the analysis of posttranslational modification as a proxy for TFEB activation are often misleading. Here, we elaborate on protocols for monitoring nuclear translocation of TFEB by fluorescence microscopy.

langue originale	Anglais
titre	Monitoring vesicular trafficking in cellular responses to stress - Part A
rédacteurs en chef	Oliver Kepp, Lorenzo Galluzzi
Editeur	Academic Press Inc.
Pages	1-9
Nombre de pages	9
ISBN (imprimé)	9780128235447
Les DOIs	https://doi.org/10.1016/bs.mcb.2020.10.017
état	Publié - 1 janv. 2021

Série de publications

Nom	Methods in Cell Biology
Volume	164
ISSN (imprimé)	0091-679X

Accès au document

10.1016/bs.mcb.2020.10.017

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Contient cette citation

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Monitoring TFEB translocation. / Chen, Guo; Mu, Chenglong; Chen, Yanfang et al.
Monitoring vesicular trafficking in cellular responses to stress - Part A. Ed. / Oliver Kepp; Lorenzo Galluzzi. Academic Press Inc., 2021. p. 1-9 (Methods in Cell Biology; Vol 164).

Résultats de recherche: Le chapitre dans un livre, un rapport, une anthologie ou une collection › !!Chapter › Revue par des pairs

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AU - Kepp, Oliver

AU - Kroemer, Guido

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