Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry

Françoise Baudouin; Marie N.Kolopp Sarda; Alain Goguel; Marie C. Béné; N. Hougardy; J. C. Capiod; F. Schillinger; F. Garnache; J. Gardais; J. Feuillard; M. Raphael; J. L. D'Hautcourt; F. Lacombe; G. Lecalvez; M. C. Léglise; V. Deneys; A. M. Mazzon; M. Bernier; V. Salaun; P. Bourin; C. Rapatel; F. Tavernier; M. Amar; P. Moskovchenko; L. Boumsell; H. Jouault; F. Lelong; E. Solary; R. O. Casasnovas; M. Maynadié; M. Favre; M. C. Jacob; G. Wallet; B. Husson; N. Lafontaine; C. Sartiaux; S. Taillefer; M. Fournier; F. Trimoreau; V. Praloran; E. Homolle; C. Brunet; P. Poncelet; J. Taib; T. Lavabre Bertrand; C. Lavabre; G. Jung; G. Kandel; R. Garand; N. Robillard; P. Philip; C. Mathiot; J. Y. Perrot; J. P. Marie; F. Valensi; V. Vergé; J. C. Homberg; G. Schaison; L. Douay; T. Samson; A. Brizard; D. Vanhaecke; S. Daliphard; B. Drenou; R. Fauchet; O. Lees; B. Lenormand; J. P. Vannier; L. Campos; O. Sabido; C. Vasselon; D. Guyotat; A. Falkenrodt; E. Kuhlein; M. H. Estienne; J. L. Bremond; M. Degenne; F. Le Baron; P. Duthilleul; G. C. Faure; M. N. Kolopp-Sarda; J. F. Lesesve; P. Bordigoni; F. Witz; C. Bayle; J. Antonini; C. Boucheix; A. Charpentier; P. Brousse; B. Chatelain; A. Dromelet

doi:10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry

Françoise Baudouin, Marie N.Kolopp Sarda, Alain Goguel, Marie C. Béné, N. Hougardy, J. C. Capiod, F. Schillinger, F. Garnache, J. Gardais, J. Feuillard, M. Raphael, J. L. D'Hautcourt, F. Lacombe, G. Lecalvez, M. C. Léglise, V. Deneys, A. M. Mazzon, M. Bernier, V. Salaun, P. BourinC. Rapatel, F. Tavernier, M. Amar, P. Moskovchenko, L. Boumsell, H. Jouault, F. Lelong, E. Solary, R. O. Casasnovas, M. Maynadié, M. Favre, M. C. Jacob, G. Wallet, B. Husson, N. Lafontaine, C. Sartiaux, S. Taillefer, M. Fournier, F. Trimoreau, V. Praloran, E. Homolle, C. Brunet, P. Poncelet, J. Taib, T. Lavabre Bertrand, C. Lavabre, G. Jung, G. Kandel, R. Garand, N. Robillard, P. Philip, C. Mathiot, J. Y. Perrot, J. P. Marie, F. Valensi, V. Vergé, J. C. Homberg, G. Schaison, L. Douay, T. Samson, A. Brizard, D. Vanhaecke, S. Daliphard, B. Drenou, R. Fauchet, O. Lees, B. Lenormand, J. P. Vannier, L. Campos, O. Sabido, C. Vasselon, D. Guyotat, A. Falkenrodt, E. Kuhlein, M. H. Estienne, J. L. Bremond, M. Degenne, F. Le Baron, P. Duthilleul, G. C. Faure, M. N. Kolopp-Sarda, J. F. Lesesve, P. Bordigoni, F. Witz, C. Bayle, J. Antonini, C. Boucheix, A. Charpentier, P. Brousse, B. Chatelain, A. Dromelet

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Résumé

The development of automated methods and normative rules, as well as the dependence of some therapeutic approaches on lymphocyte subsets counts, has led to the appearance of calibration reagents. Such reagents are expected to perform equally well in very different settings. We developed a multicenter trial to evaluate the performance of a new quality control reagent, i.e., stabilized blood to be used in immunophenotyping laboratories. Aliquots of the same batch of stabilized blood were shipped to 45 French and Belgian laboratories on a Monday and had to be tested for percentages and absolute counts of at least CD3-, CD4-, and CD8-positive lymphocytes on 4 consecutive days. The percentages and absolute counts obtained on each assay were recorded, as well as the type of lysis used, the trademark of reagents, and the brand of flow cytometer. The mean values collected did not differ significantly from those expected by the manufacturer. Absolute counts generated through one-step techniques displayed lower CVs. This new reagent therefore appears to be a robust product, liable to yield consistent results in the array of different conditions represented by routine laboratories, and it could be useful for quality control procedures.

langue originale	Anglais
Pages (de - à)	127-132
Nombre de pages	6
journal	Communications in Clinical Cytometry
Volume	38
Numéro de publication	3
Les DOIs	https://doi.org/10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B
état	Publié - 15 juin 1999

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10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

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Baudouin, F., Sarda, M. N. K., Goguel, A., Béné, M. C., Hougardy, N., Capiod, J. C., Schillinger, F., Garnache, F., Gardais, J., Feuillard, J., Raphael, M., D'Hautcourt, J. L., Lacombe, F., Lecalvez, G., Léglise, M. C., Deneys, V., Mazzon, A. M., Bernier, M., Salaun, V., ... Dromelet, A. (1999). Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry. Communications in Clinical Cytometry, 38(3), 127-132. https://doi.org/10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

@article{f4c2e3825e164b5eb83cf7c696a651d6,

title = "Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry",

abstract = "The development of automated methods and normative rules, as well as the dependence of some therapeutic approaches on lymphocyte subsets counts, has led to the appearance of calibration reagents. Such reagents are expected to perform equally well in very different settings. We developed a multicenter trial to evaluate the performance of a new quality control reagent, i.e., stabilized blood to be used in immunophenotyping laboratories. Aliquots of the same batch of stabilized blood were shipped to 45 French and Belgian laboratories on a Monday and had to be tested for percentages and absolute counts of at least CD3-, CD4-, and CD8-positive lymphocytes on 4 consecutive days. The percentages and absolute counts obtained on each assay were recorded, as well as the type of lysis used, the trademark of reagents, and the brand of flow cytometer. The mean values collected did not differ significantly from those expected by the manufacturer. Absolute counts generated through one-step techniques displayed lower CVs. This new reagent therefore appears to be a robust product, liable to yield consistent results in the array of different conditions represented by routine laboratories, and it could be useful for quality control procedures.",

keywords = "Absolute counts, CD4, Flow cytometry, Lymphocyte subsets, Peripheral blood, Quality control",

author = "Fran{\c c}oise Baudouin and Sarda, {Marie N.Kolopp} and Alain Goguel and B{\'e}n{\'e}, {Marie C.} and N. Hougardy and Capiod, {J. C.} and F. Schillinger and F. Garnache and J. Gardais and J. Feuillard and M. Raphael and D'Hautcourt, {J. L.} and F. Lacombe and G. Lecalvez and L{\'e}glise, {M. C.} and V. Deneys and Mazzon, {A. M.} and M. Bernier and V. Salaun and P. Bourin and C. Rapatel and F. Tavernier and M. Amar and P. Moskovchenko and L. Boumsell and H. Jouault and F. Lelong and E. Solary and Casasnovas, {R. O.} and M. Maynadi{\'e} and M. Favre and Jacob, {M. C.} and G. Wallet and B. Husson and N. Lafontaine and C. Sartiaux and S. Taillefer and M. Fournier and F. Trimoreau and V. Praloran and E. Homolle and C. Brunet and P. Poncelet and J. Taib and {Lavabre Bertrand}, T. and C. Lavabre and G. Jung and G. Kandel and R. Garand and N. Robillard and P. Philip and C. Mathiot and Perrot, {J. Y.} and Marie, {J. P.} and F. Valensi and V. Verg{\'e} and Homberg, {J. C.} and G. Schaison and L. Douay and T. Samson and A. Brizard and D. Vanhaecke and S. Daliphard and B. Drenou and R. Fauchet and O. Lees and B. Lenormand and Vannier, {J. P.} and L. Campos and O. Sabido and C. Vasselon and D. Guyotat and A. Falkenrodt and E. Kuhlein and Estienne, {M. H.} and Bremond, {J. L.} and M. Degenne and {Le Baron}, F. and P. Duthilleul and Faure, {G. C.} and Kolopp-Sarda, {M. N.} and Lesesve, {J. F.} and P. Bordigoni and F. Witz and C. Bayle and J. Antonini and C. Boucheix and A. Charpentier and P. Brousse and B. Chatelain and A. Dromelet",

year = "1999",

month = jun,

day = "15",

doi = "10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B",

language = "English",

volume = "38",

pages = "127--132",

journal = "Communications in Clinical Cytometry",

issn = "0196-4763",

number = "3",

}

Baudouin, F, Sarda, MNK, Goguel, A, Béné, MC, Hougardy, N, Capiod, JC, Schillinger, F, Garnache, F, Gardais, J, Feuillard, J, Raphael, M, D'Hautcourt, JL, Lacombe, F, Lecalvez, G, Léglise, MC, Deneys, V, Mazzon, AM, Bernier, M, Salaun, V, Bourin, P, Rapatel, C, Tavernier, F, Amar, M, Moskovchenko, P, Boumsell, L, Jouault, H, Lelong, F, Solary, E, Casasnovas, RO, Maynadié, M, Favre, M, Jacob, MC, Wallet, G, Husson, B, Lafontaine, N, Sartiaux, C, Taillefer, S, Fournier, M, Trimoreau, F, Praloran, V, Homolle, E, Brunet, C, Poncelet, P, Taib, J, Lavabre Bertrand, T, Lavabre, C, Jung, G, Kandel, G, Garand, R, Robillard, N, Philip, P, Mathiot, C, Perrot, JY, Marie, JP, Valensi, F, Vergé, V, Homberg, JC, Schaison, G, Douay, L, Samson, T, Brizard, A, Vanhaecke, D, Daliphard, S, Drenou, B, Fauchet, R, Lees, O, Lenormand, B, Vannier, JP, Campos, L, Sabido, O, Vasselon, C, Guyotat, D, Falkenrodt, A, Kuhlein, E, Estienne, MH, Bremond, JL, Degenne, M, Le Baron, F, Duthilleul, P, Faure, GC, Kolopp-Sarda, MN, Lesesve, JF, Bordigoni, P, Witz, F, Bayle, C, Antonini, J, Boucheix, C, Charpentier, A, Brousse, P, Chatelain, B & Dromelet, A 1999, 'Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry', Communications in Clinical Cytometry, VOL. 38, Numéro 3, p. 127-132. https://doi.org/10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

TY - JOUR

T1 - Multicenter study of reference stabilized human blood for lymphocyte immunophenotyping quality control in flow cytometry

AU - Baudouin, Françoise

AU - Sarda, Marie N.Kolopp

AU - Goguel, Alain

AU - Béné, Marie C.

AU - Hougardy, N.

AU - Capiod, J. C.

AU - Schillinger, F.

AU - Garnache, F.

AU - Gardais, J.

AU - Feuillard, J.

AU - Raphael, M.

AU - D'Hautcourt, J. L.

AU - Lacombe, F.

AU - Lecalvez, G.

AU - Léglise, M. C.

AU - Deneys, V.

AU - Mazzon, A. M.

AU - Bernier, M.

AU - Salaun, V.

AU - Bourin, P.

AU - Rapatel, C.

AU - Tavernier, F.

AU - Amar, M.

AU - Moskovchenko, P.

AU - Boumsell, L.

AU - Jouault, H.

AU - Lelong, F.

AU - Solary, E.

AU - Casasnovas, R. O.

AU - Maynadié, M.

AU - Favre, M.

AU - Jacob, M. C.

AU - Wallet, G.

AU - Husson, B.

AU - Lafontaine, N.

AU - Sartiaux, C.

AU - Taillefer, S.

AU - Fournier, M.

AU - Trimoreau, F.

AU - Praloran, V.

AU - Homolle, E.

AU - Brunet, C.

AU - Poncelet, P.

AU - Taib, J.

AU - Lavabre Bertrand, T.

AU - Lavabre, C.

AU - Jung, G.

AU - Kandel, G.

AU - Garand, R.

AU - Robillard, N.

AU - Philip, P.

AU - Mathiot, C.

AU - Perrot, J. Y.

AU - Marie, J. P.

AU - Valensi, F.

AU - Vergé, V.

AU - Homberg, J. C.

AU - Schaison, G.

AU - Douay, L.

AU - Samson, T.

AU - Brizard, A.

AU - Vanhaecke, D.

AU - Daliphard, S.

AU - Drenou, B.

AU - Fauchet, R.

AU - Lees, O.

AU - Lenormand, B.

AU - Vannier, J. P.

AU - Campos, L.

AU - Sabido, O.

AU - Vasselon, C.

AU - Guyotat, D.

AU - Falkenrodt, A.

AU - Kuhlein, E.

AU - Estienne, M. H.

AU - Bremond, J. L.

AU - Degenne, M.

AU - Le Baron, F.

AU - Duthilleul, P.

AU - Faure, G. C.

AU - Kolopp-Sarda, M. N.

AU - Lesesve, J. F.

AU - Bordigoni, P.

AU - Witz, F.

AU - Bayle, C.

AU - Antonini, J.

AU - Boucheix, C.

AU - Charpentier, A.

AU - Brousse, P.

AU - Chatelain, B.

AU - Dromelet, A.

PY - 1999/6/15

Y1 - 1999/6/15

N2 - The development of automated methods and normative rules, as well as the dependence of some therapeutic approaches on lymphocyte subsets counts, has led to the appearance of calibration reagents. Such reagents are expected to perform equally well in very different settings. We developed a multicenter trial to evaluate the performance of a new quality control reagent, i.e., stabilized blood to be used in immunophenotyping laboratories. Aliquots of the same batch of stabilized blood were shipped to 45 French and Belgian laboratories on a Monday and had to be tested for percentages and absolute counts of at least CD3-, CD4-, and CD8-positive lymphocytes on 4 consecutive days. The percentages and absolute counts obtained on each assay were recorded, as well as the type of lysis used, the trademark of reagents, and the brand of flow cytometer. The mean values collected did not differ significantly from those expected by the manufacturer. Absolute counts generated through one-step techniques displayed lower CVs. This new reagent therefore appears to be a robust product, liable to yield consistent results in the array of different conditions represented by routine laboratories, and it could be useful for quality control procedures.

AB - The development of automated methods and normative rules, as well as the dependence of some therapeutic approaches on lymphocyte subsets counts, has led to the appearance of calibration reagents. Such reagents are expected to perform equally well in very different settings. We developed a multicenter trial to evaluate the performance of a new quality control reagent, i.e., stabilized blood to be used in immunophenotyping laboratories. Aliquots of the same batch of stabilized blood were shipped to 45 French and Belgian laboratories on a Monday and had to be tested for percentages and absolute counts of at least CD3-, CD4-, and CD8-positive lymphocytes on 4 consecutive days. The percentages and absolute counts obtained on each assay were recorded, as well as the type of lysis used, the trademark of reagents, and the brand of flow cytometer. The mean values collected did not differ significantly from those expected by the manufacturer. Absolute counts generated through one-step techniques displayed lower CVs. This new reagent therefore appears to be a robust product, liable to yield consistent results in the array of different conditions represented by routine laboratories, and it could be useful for quality control procedures.

KW - Absolute counts

KW - CD4

KW - Flow cytometry

KW - Lymphocyte subsets

KW - Peripheral blood

KW - Quality control

UR - http://www.scopus.com/inward/record.url?scp=0033564224&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

DO - 10.1002/(SICI)1097-0320(19990615)38:3<127::AID-CYTO7>3.0.CO;2-B

M3 - Article

C2 - 10397332

AN - SCOPUS:0033564224

SN - 0196-4763

VL - 38

SP - 127

EP - 132

JO - Communications in Clinical Cytometry

JF - Communications in Clinical Cytometry

IS - 3

ER -