Nanosecond-duration electric pulses open nanometer-size pores in cell plasma membrane

A. G. Pakhomov, B. L. Ibey, A. M. Bowman, F. M. Andre, O. N. Pakhomova

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Résumé

We found that ultra-short electric pulses (60 or 600 ns duration) applied to mammalian cells cause profound, dose-dependent increase of plasma membrane electrical conductance. This effect is detectable even minutes after the exposure and is explained by formation of long-lived, voltagesensitive, inward-rectifying pores of nanometer diameter ("nanopores"). The phenomenon of nanoelectroporation and the extended lifetime of nanopores can also be demonstrated by a surge of Tl+ uptake in the presence of K+ channel blockers, as well as in CHO cells that express no endogenous voltage-gated K+ channels. Due to their long lifetime, nanopores can have significant impact on cell physiology.

langue originaleAnglais
titreWorld Congress on Medical Physics and Biomedical Engineering
Sous-titreSpecial Topics and Workshops
EditeurSpringer Verlag
Pages17-20
Nombre de pages4
Edition13
ISBN (imprimé)9783642038945
Les DOIs
étatPublié - 1 janv. 2009
Modification externeOui
EvénementWorld Congress on Medical Physics and Biomedical Engineering: Special Topics and Workshops - Munich, Allemagne
Durée: 7 sept. 200912 sept. 2009

Série de publications

NomIFMBE Proceedings
nombre13
Volume25
ISSN (imprimé)1680-0737

Une conférence

Une conférenceWorld Congress on Medical Physics and Biomedical Engineering: Special Topics and Workshops
Pays/TerritoireAllemagne
La villeMunich
période7/09/0912/09/09

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