Nonsense mutations inhibit RNA splicing in a cell-free system: Recognition of mutant codon is independent of protein synthesis

Said Aoufouchi, José Yélamos, César Milstein

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

126 Citations (Scopus)

Résumé

Mutations resulting in premature termination codons reduce the corresponding mRNA levels. We describe a cell-free system in which depletion of the mutant immunoglobulin κ mRNA pool correlates with inefficient splicing and not with RNA decay. Splicing deficiency does not depend on the sequence surrounding the in-frame nonsense codon and can be partially corrected by mutating the methionine initiation codon. Despite the apparent link between translation and low mutant mRNA levels, inefficient splicing is not dependent on protein synthesis. Abnormal splicing of mutant immunoglobulin RNA is observed with R-cell but not with HeLa or T-cell extracts. A nonsense mutant β-globin RNA is normally spliced by S-cell extract. We propose that the phenomenon exhibits tissue and gene specificity.

langue originaleAnglais
Pages (de - à)415-422
Nombre de pages8
journalCell
Volume85
Numéro de publication3
Les DOIs
étatPublié - 3 mai 1996
Modification externeOui

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