Résumé
Mutations resulting in premature termination codons reduce the corresponding mRNA levels. We describe a cell-free system in which depletion of the mutant immunoglobulin κ mRNA pool correlates with inefficient splicing and not with RNA decay. Splicing deficiency does not depend on the sequence surrounding the in-frame nonsense codon and can be partially corrected by mutating the methionine initiation codon. Despite the apparent link between translation and low mutant mRNA levels, inefficient splicing is not dependent on protein synthesis. Abnormal splicing of mutant immunoglobulin RNA is observed with R-cell but not with HeLa or T-cell extracts. A nonsense mutant β-globin RNA is normally spliced by S-cell extract. We propose that the phenomenon exhibits tissue and gene specificity.
langue originale | Anglais |
---|---|
Pages (de - à) | 415-422 |
Nombre de pages | 8 |
journal | Cell |
Volume | 85 |
Numéro de publication | 3 |
Les DOIs | |
état | Publié - 3 mai 1996 |
Modification externe | Oui |