TY - JOUR
T1 - Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit
AU - Heidmann, O.
AU - Auffray, C.
AU - Cazenave, P. A.
AU - Rougeon, F.
PY - 1981/1/1
Y1 - 1981/1/1
N2 - A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated form the spleen and translated in vitro, and translation products have been analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Double-stranded cDNa has been synthesized from poly(A)-containing RNA template and inserted in the Pst I endonuclease site of plasmid pBR322 by using the oligo(dC)oligo(dG) tailing procedure. Tetracycline-resistant ampicillin-sensitive clones containing cDNA complementary to a κ light chain mRNA have been selected by differential screening and their ability to hyrbidize to a spleen mRNA having the same size as a mouse κ light chain mRNA. Two clones, pRk-15 and pRk 32, have been selected to determine the nucleotide sequence of the constant and 3' untranslated regions of κ light chain mRNA, by the Maxam and Gilbert partial degradation method. Comparison of homologous regions of mouse κ chain mRNA and b4 rabbit κ chain mRNA reveals 61% homology in the constant region and 59% homology in the 3' untranslated region.
AB - A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated form the spleen and translated in vitro, and translation products have been analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Double-stranded cDNa has been synthesized from poly(A)-containing RNA template and inserted in the Pst I endonuclease site of plasmid pBR322 by using the oligo(dC)oligo(dG) tailing procedure. Tetracycline-resistant ampicillin-sensitive clones containing cDNA complementary to a κ light chain mRNA have been selected by differential screening and their ability to hyrbidize to a spleen mRNA having the same size as a mouse κ light chain mRNA. Two clones, pRk-15 and pRk 32, have been selected to determine the nucleotide sequence of the constant and 3' untranslated regions of κ light chain mRNA, by the Maxam and Gilbert partial degradation method. Comparison of homologous regions of mouse κ chain mRNA and b4 rabbit κ chain mRNA reveals 61% homology in the constant region and 59% homology in the 3' untranslated region.
UR - http://www.scopus.com/inward/record.url?scp=0019815523&partnerID=8YFLogxK
U2 - 10.1073/pnas.78.9.5802
DO - 10.1073/pnas.78.9.5802
M3 - Article
C2 - 6795636
AN - SCOPUS:0019815523
SN - 0027-8424
VL - 78
SP - 5802
EP - 5806
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9 II
ER -