Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade

Giulia Cerrato, Marion Leduc, Kevin Müller, Peng Liu, Liwei Zhao, Juliette Humeau, Wei Xie, Shuai Zhang, Oliver Kepp, Allan Sauvat, Guido Kroemer

    Résultats de recherche: Contribution à un journalArticleRevue par des pairs

    7 Citations (Scopus)

    Résumé

    Oleate, the most abundant endogenous and dietary cis-unsaturated fatty acid, has the atypical property to cause the redistribution of microtubule-associated proteins 1A/1B light chain 3B (referred to as LC3) to the trans-Golgi network (TGN), as shown here. A genome-wide screen identified multiple, mostly Golgi transport-related genes specifically involved in the oleate-induced relocation of LC3 to the Golgi apparatus. Follow-up analyses revealed that oleate also caused the retention of secreted proteins in the TGN, as determined in two assays in which the secretion of proteins was synchronized, (i) an assay involving a thermosensitive vesicular stomatitis virus G (VSVG) protein that is retained in the endoplasmic reticulum (ER) until the temperature is lowered, and (ii) an isothermic assay involving the reversible retention of the protein of interest in the ER lumen and that was used both in vitro and in vivo. A pharmacological screen searching for agents that induce LC3 aggregation at the Golgi apparatus led to the identification of “oleate mimetics” that share the capacity to block conventional protein secretion. In conclusion, oleate represents a class of molecules that act on the Golgi apparatus to cause the recruitment of LC3 and to stall protein secretion.

    langue originaleAnglais
    Pages (de - à)1733-1752
    Nombre de pages20
    journalCell Death and Differentiation
    Volume28
    Numéro de publication5
    Les DOIs
    étatPublié - 1 mai 2021

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