@inbook{647c5b921f4c4305acd94b023fd6ffed,
title = "Oncogene-Driven Induction of Orthotopic Cholangiocarcinoma in Mice",
abstract = "Cholangiocarcinoma (CCA) is a malignancy affecting the epithelial cells that line the bile ducts. This cancer shows a poor prognosis and current treatments remain inefficient. Orthotopic CCA mouse models are useful for the development of innovative therapeutic strategies. Here, we describe an orthotopic model of intrahepatic CCA that can be easily induced in mice within 5 weeks at a high incidence. It is achieved by expressing two oncogenes, namely, (i) the intracellular domain of the Notch1 receptor (NICD) and (ii) AKT, in hepatocytes by means of the sleeping beauty transposon system. These plasmid vectors are delivered by hydrodynamic injection into the tail vein. The present chapter also describes how to perform magnetic resonance imaging (MRI) of the livers to visualize intrahepatic CCA nodules.",
keywords = "Bile duct cancer, Cholangiocarcinoma, Hydrodynamic injection, Magnetic resonance imaging, Orthotopic model",
author = "C{\'e}leste Plantureux and Juliette Paillet and Gwennhael Autret and Maria P{\'e}rez-Lanz{\'o}n and Guido Kroemer and Maiuri, {Maria Chiara} and Jonathan Pol",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
month = jan,
day = "1",
doi = "10.1007/978-1-0716-3694-7_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "99--108",
booktitle = "Methods in Molecular Biology",
}