Peptoniphilus genitalis sp. nov. and Mobiluncus massiliensis sp. nov. Novel Bacteria Isolated from the Vaginal Microbiome

Linda Abou Chacra, Marion Bonnet, Mégane Heredia, Gabriel Haddad, Nicholas Armstrong, Stéphane Alibar, Florence Bretelle, Florence Fenollar

Résultats de recherche: Contribution à un journalArticleRevue par des pairs

Résumé

The strains Marseille-Q7072T (= CSUR Q7072T = CECT 30604 T) and Marseille-Q7826T (= CSUR Q7826T = CECT 30727 T) were isolated from vaginal samples. As MALDI-TOF mass spectrometry failed to identify them, their genomes were directly sequenced to determine their taxogenomic identities. Both strains are anaerobic without any oxidase and catalase activity. C16:0 is the most abundant fatty acid for both strains. Strain Marseille-Q7072T is non-spore-forming, non-motile, Gram-stain-positive, and coccus-shaped, while strain Marseille-Q7826T is non-spore-forming, motile, Gram-stain-variable, and curved rod-shaped. The genomic comparison of the Marseille-Q7072T and Marseille-Q7826T strains showed that all digital DNA–DNA hybridisation (dDDH) and mean orthologous nucleotide identity (OrthoANI) values were below published species thresholds (70% and 95–96%, respectively) with other closely related species with standing in nomenclature. Thus, we conclude that both strains are new bacterial species. Strain Marseille-Q7072T is a new member of the Bacillota phylum, for which the name Peptoniphilus genitalis sp. nov. is proposed, while the Marseille-Q7826T strain is a new member of the Actinomycetota phylum, for which the name Mobiluncus massiliensis sp. nov. is proposed.

langue originaleAnglais
Numéro d'article97
journalCurrent Microbiology
Volume81
Numéro de publication4
Les DOIs
étatPublié - 1 avr. 2024
Modification externeOui

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