TY - JOUR
T1 - Prognostic Value of Fusobacterium nucleatum after Abdominoperineal Resection for Anal Squamous Cell Carcinoma
AU - Hilmi, Marc
AU - Neuzillet, Cindy
AU - Lefèvre, Jérémie H.
AU - Svrcek, Magali
AU - Vacher, Sophie
AU - Benhaim, Leonor
AU - Dartigues, Peggy
AU - Samalin, Emmanuelle
AU - Lazartigues, Julien
AU - Emile, Jean François
AU - Rigault, Eugénie
AU - Rioux-Leclercq, Nathalie
AU - de La Fouchardière, Christelle
AU - Tougeron, David
AU - Cacheux, Wulfran
AU - Mariani, Pascale
AU - Courtois, Laura
AU - Delaye, Matthieu
AU - Dangles-Marie, Virginie
AU - Lièvre, Astrid
AU - Bieche, Ivan
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Main prognostic factors of anal squamous cell carcinoma (ASCC) are tumor size, differ-entiation, lymph node involvement, and male gender. However, they are insufficient to predict relapses after exclusive radiotherapy (RT) or chemoradiotherapy (CRT). Fusobacterium nucleatum has been associated with poor prognosis in several digestive cancers. In this study, we assessed the association between intratumoral F. nucleatum load and clinico-pathological features, relapse, and survival in patients with ASCC who underwent abdominoperineal resection (APR) after RT/CRT. We retrospectively analyzed surgical samples from a cohort of 166 patients with ASCC who underwent APR. F. nucleatum 16S rRNA gene sequences were quantified using real-time quantitative PCR. We associated F. nucleatum load with classical clinicopathological features, overall survival (OS), disease-free survival (DFS), and metastasis-free survival (MFS) using Cox regression univariate and multivariate analyses. Tumors harboring high loads of F. nucleatum (highest tercile) showed longer OS and DFS (median: not reached vs. 50.1 months, p = 0.01, and median: not reached vs. 18.3 months, p = 0.007, respectively). High F. nucleatum load was a predictor of longer OS (HR = 0.55, p = 0.04) and DFS (HR = 0.50, p = 0.02) in multivariate analysis. High F. nucleatum load is an independent favorable prognostic factor in patients with ASCC who underwent APR.
AB - Main prognostic factors of anal squamous cell carcinoma (ASCC) are tumor size, differ-entiation, lymph node involvement, and male gender. However, they are insufficient to predict relapses after exclusive radiotherapy (RT) or chemoradiotherapy (CRT). Fusobacterium nucleatum has been associated with poor prognosis in several digestive cancers. In this study, we assessed the association between intratumoral F. nucleatum load and clinico-pathological features, relapse, and survival in patients with ASCC who underwent abdominoperineal resection (APR) after RT/CRT. We retrospectively analyzed surgical samples from a cohort of 166 patients with ASCC who underwent APR. F. nucleatum 16S rRNA gene sequences were quantified using real-time quantitative PCR. We associated F. nucleatum load with classical clinicopathological features, overall survival (OS), disease-free survival (DFS), and metastasis-free survival (MFS) using Cox regression univariate and multivariate analyses. Tumors harboring high loads of F. nucleatum (highest tercile) showed longer OS and DFS (median: not reached vs. 50.1 months, p = 0.01, and median: not reached vs. 18.3 months, p = 0.007, respectively). High F. nucleatum load was a predictor of longer OS (HR = 0.55, p = 0.04) and DFS (HR = 0.50, p = 0.02) in multivariate analysis. High F. nucleatum load is an independent favorable prognostic factor in patients with ASCC who underwent APR.
KW - Anal squamous cell carcinoma
KW - Cancer microenvironment
KW - Fusobacterium nucleatum
KW - Intratumoral microbiota
KW - Tumor biomarkers
UR - http://www.scopus.com/inward/record.url?scp=85126978013&partnerID=8YFLogxK
U2 - 10.3390/cancers14071606
DO - 10.3390/cancers14071606
M3 - Article
AN - SCOPUS:85126978013
SN - 2072-6694
VL - 14
JO - Cancers
JF - Cancers
IS - 7
M1 - 1606
ER -