@inbook{ac58c5a105d840b2b2b81ca2a706057e,
title = "Quantification of cell cycle-arresting proteins",
abstract = "Cellular senescence, which can be defined as a stress response preventing the propagation of cells that have accumulated potentially oncogenic alterations, is invariably associated with a permanent cell cycle arrest. Such an irreversible blockage is mainly mediated by the persistent upregulation of one or more cyclindependent kinase inhibitors (CKIs), including (though not limited to) p16INK4A and p21CIP1 and p27KIP1. CKIs operate by binding to cyclin-dependent kinases (CDKs), de facto inhibiting their enzymatic activity. Here, we provide an immunoblotting-based method for the detection and quantification of CKIs in vitro and ex vivo, together with a set of guidelines for the interpretation of results.",
keywords = "ARF, Cancer, DNA damage, INK",
author = "Oliver Kepp and Isabelle Martins and Laurie Menger and Micka{\"e}l Michaud and Sandy Adjemian and Sukkurwala, {Abdul Qader} and Lorenzo Galluzzi and Guido Kroemer",
year = "2013",
month = jan,
day = "1",
doi = "10.1007/978-1-62703-239-1_7",
language = "English",
isbn = "9781627032384",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "121--142",
booktitle = "Cell Senescence",
}